Custom Protein Expression & Purification

* Please be kindly noted that our services and products can only be used for research to organizations or companies and not intended for any clinical or individuals.

Recombinant protein expression can be used in all areas of life science research, from cell protein analysis to high-yield protein production and drug development. BOC Sciences provides customized and reliable solutions that can quickly express and purify any protein to meet your exact specifications.

Why Choose Us?

  • Comprehensive expression system: Providing a variety of vectors including bacteria, yeast, baculovirus-insect, and mammalian expression systems
  • One-stop service from gene to protein
  • High-throughput and large-scale production, with capacities ranging from 2 liters to 1000 liters
  • Provide multiple purification systems
  • Post-purification services: desalination, aliquoting, endotoxin removal, aseptic process, and lyophilization
  • Dedicated and experienced project management team

Protein Expression & Purification Systems

  • Gene synthesis and codon optimization (optional)
  • Vector construction
  • Expression evaluation
  • Protein expression and purification (large-scale also available)
  • QC by SDS-PAGE, Western blot
  • Gene synthesis and codon optimization (optional)
  • Vector construction
  • Expression evaluation
  • Large-scale expression
  • Purification
  • QC by SDS-PAGE, Western blot
Strain UsedE.coliPichia pastoris, Saccharomyces cerevisiae
  • Relatively inexpensive
  • Easy to manipulate
  • Easily scale-up
  • Fast expression
  • Diverse post-translational modifications
  • Industry-scale fermentation
  • Lack of effective post-translational modification
  • Inclusion body
  • Low yields and low activity of some eukaryotic proteins
  • Difficult to express proteins of high molecular weight
Improper and/or excessive glycosylation
  • Gene synthesis and codon optimization (optional)
  • Vector construction
  • Virus packaging and amplification
  • Expression and purification (large-scale also available)
  • QC by SDS-PAGE, Western blot
  • Gene synthesis and codon optimization (optional)
  • Vector construction
  • Stable cell line development/construction (for stable protein expression)
  • Expression and purification (Large-scale also available)
  • QC by SDS-PAGE, Western blot
Strain UsedSf-9, Sf-21, S-2, Hi-5, etc.CHO, HEK-293 etc.
  • Ability to integrate post-translational modifications
  • Can produce a large amount of target protein
  • Cost-effective compared with other eukaryotic expression systems
  • Comprehensive post-translational modifications
  • The best method to produce bioactive proteins
  • Transient and stable protein expressions
  • Long production time
  • Long production time
  • Relative low protein yields

Other expression services

  • Lyophilization services
  • Fermentation services
  • cGMP manufacturing services

Purification and Quality Control

  • Purification methods we use include
    • Affinity chromatography
    • Size Exclusion Chromatography (SEC)
    • Ion Exchange Chromatography (IEX)
  • We provide a variety of affinity tags
    • His-tag
    • Fc-tag (Protein A/G)
    • Strep-tag
    • GST-label

According to demand, we can provide:

  • Label cutting
  • Analysis SEC (HPLC)
  • Custom buffer
  • Endotoxin determination
  • Repacking service
  • Density adjustment

Our standard quality control methods include

  • SDS-PAGE, Coomassie brilliant blue protein staining
  • Spectrophotometric determination of protein concentration
  • Western blot analysis of tag-specific antibodies

Upon request we can also provide:

  • Capillary electrophoresis, detection by UV absorption and/or fluorescence
  • Analysis SEC (HPLC)
  • LAL method for determination of endotoxin

Service Process

Service Process

BOC Sciences has been a recognized leader in the industry in providing high-quality recombinant protein expression and purification services. We utilize a variety of improved technology platforms to provide the best services. For more information, please contact us.

* PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.

BOC Sciences Support

Please contact us with any specific requirements and we will get back to you as soon as possible.

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