Custom Protein Expression & Purification
Recombinant protein expression can be used in all areas of life science research, from cell protein analysis to high-yield protein production and drug development. BOC Sciences provides customized and reliable solutions that can quickly express and purify any protein to meet your exact specifications.
Why Choose Us?
- Comprehensive expression system: Providing a variety of vectors including bacteria, yeast, baculovirus-insect, and mammalian expression systems
- One-stop service from gene to protein
- High-throughput and large-scale production, with capacities ranging from 2 liters to 1000 liters
- Provide multiple purification systems
- Post-purification services: desalination, aliquoting, endotoxin removal, aseptic process, and lyophilization
- Dedicated and experienced project management team
Protein Expression & Purification Systems
| System | Bacterial | Yeast |
|---|
| Services | - Gene synthesis and codon optimization (optional)
- Vector construction
- Expression evaluation
- Protein expression and purification (large-scale also available)
- QC by SDS-PAGE, Western blot
| - Gene synthesis and codon optimization (optional)
- Vector construction
- Expression evaluation
- Large-scale expression
- Purification
- QC by SDS-PAGE, Western blot
|
| Strain Used | E.coli | Pichia pastoris, Saccharomyces cerevisiae |
| Pros | - Relatively inexpensive
- Easy to manipulate
- Easily scale-up
- Fast expression
| - Diverse post-translational modifications
- Industry-scale fermentation
|
| Cons | - Lack of effective post-translational modification
- Inclusion body
- Low yields and low activity of some eukaryotic proteins
- Difficult to express proteins of high molecular weight
| Improper and/or excessive glycosylation
glycosylation |
| System | Baculovirus-insect | Mammalian |
|---|
| Services | - Gene synthesis and codon optimization (optional)
- Vector construction
- Virus packaging and amplification
- Expression and purification (large-scale also available)
- QC by SDS-PAGE, Western blot
| - Gene synthesis and codon optimization (optional)
- Vector construction
- Stable cell line development/construction (for stable protein expression)
- Expression and purification (Large-scale also available)
- QC by SDS-PAGE, Western blot
|
| Strain Used | Sf-9, Sf-21, S-2, Hi-5, etc. | CHO, HEK-293 etc. |
| Pros | - Ability to integrate post-translational modifications
- Can produce a large amount of target protein
- Cost-effective compared with other eukaryotic expression systems
| - Comprehensive post-translational modifications
- The best method to produce bioactive proteins
- Transient and stable protein expressions
|
| Cons | | - Long production time
- Relative low protein yields
|
Other expression services
- Lyophilization services
- Fermentation services
- cGMP manufacturing services
Purification and Quality Control
- Purification methods we use include
- Affinity chromatography
- Size Exclusion Chromatography (SEC)
- Ion Exchange Chromatography (IEX)
- We provide a variety of affinity tags
- His-tag
- Fc-tag (Protein A/G)
- Strep-tag
- GST-label
According to demand, we can provide:
- Label cutting
- Analysis SEC (HPLC)
- Custom buffer
- Endotoxin determination
- Repacking service
- Density adjustment
Our standard quality control methods include
- SDS-PAGE, Coomassie brilliant blue protein staining
- Spectrophotometric determination of protein concentration
- Western blot analysis of tag-specific antibodies
Upon request we can also provide:
- Capillary electrophoresis, detection by UV absorption and/or fluorescence
- Analysis SEC (HPLC)
- LAL method for determination of endotoxin
Service Process
BOC Sciences has been a recognized leader in the industry in providing high-quality recombinant protein expression and purification services. We utilize a variety of improved technology platforms to provide the best services. For more information, please contact us.
* PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.
