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Custom Protein Expression & Purification

Custom Protein Expression & Purification

Name: Custom Protein Expression & Purification
Brand: BOC Sciences

* Please be kindly noted that our services and products can only be used for research to organizations or companies and not intended for any clinical or individuals.

Consult with Our Experts

Recombinant protein expression can be used in all areas of life science research, from cell protein analysis to high-yield protein production and drug development. BOC Sciences provides customized and reliable solutions that can quickly express and purify any protein to meet your exact specifications.

Why Choose Us?

Comprehensive expression system: Providing a variety of vectors including bacteria, yeast, baculovirus-insect, and mammalian expression systems
One-stop service from gene to protein
High-throughput and large-scale production, with capacities ranging from 2 liters to 1000 liters
Provide multiple purification systems
Post-purification services: desalination, aliquoting, endotoxin removal, aseptic process, and lyophilization
Dedicated and experienced project management team

Protein Expression & Purification Systems

System	Bacterial	Yeast
Services	Gene synthesis and codon optimization (optional) Vector construction Expression evaluation Protein expression and purification (large-scale also available) QC by SDS-PAGE, Western blot	Gene synthesis and codon optimization (optional) Vector construction Expression evaluation Large-scale expression Purification QC by SDS-PAGE, Western blot
Strain Used	E.coli	Pichia pastoris, Saccharomyces cerevisiae
Pros	Relatively inexpensive Easy to manipulate Easily scale-up Fast expression	Diverse post-translational modifications Industry-scale fermentation
Cons	Lack of effective post-translational modification Inclusion body Low yields and low activity of some eukaryotic proteins Difficult to express proteins of high molecular weight	Improper and/or excessive glycosylation glycosylation

System	Baculovirus-insect	Mammalian
Services	Gene synthesis and codon optimization (optional) Vector construction Virus packaging and amplification Expression and purification (large-scale also available) QC by SDS-PAGE, Western blot	Gene synthesis and codon optimization (optional) Vector construction Stable cell line development/construction (for stable protein expression) Expression and purification (Large-scale also available) QC by SDS-PAGE, Western blot
Strain Used	Sf-9, Sf-21, S-2, Hi-5, etc.	CHO, HEK-293 etc.
Pros	Ability to integrate post-translational modifications Can produce a large amount of target protein Cost-effective compared with other eukaryotic expression systems	Comprehensive post-translational modifications The best method to produce bioactive proteins Transient and stable protein expressions
Cons	Long production time	Long production time Relative low protein yields

Other expression services

Lyophilization services
Fermentation services
cGMP manufacturing services

Purification and Quality Control

Purification methods we use include
- Affinity chromatography
- Size Exclusion Chromatography (SEC)
- Ion Exchange Chromatography (IEX)
We provide a variety of affinity tags
- His-tag
- Fc-tag (Protein A/G)
- GST-label

According to demand, we can provide:

Label cutting
Analysis SEC (HPLC)
Custom buffer
Endotoxin determination
Repacking service
Density adjustment

Our standard quality control methods include

SDS-PAGE, Coomassie brilliant blue protein staining
Spectrophotometric determination of protein concentration
Western blot analysis of tag-specific antibodies

Upon request we can also provide:

Capillary electrophoresis, detection by UV absorption and/or fluorescence
Analysis SEC (HPLC)
LAL method for determination of endotoxin

Service Process

BOC Sciences has been a recognized leader in the industry in providing high-quality recombinant protein expression and purification services. We utilize a variety of improved technology platforms to provide the best services. For more information, please contact us.

* PROTAC^® is a registered trademark of Arvinas Operations, Inc., and is used under license.

BOC Sciences Support

Please contact us with any specific requirements and we will get back to you as soon as possible.

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