* Please be kindly noted that our services and products can only be used for research to organizations or companies and not intended for any clinical or individuals.
Ubiquitylation is an important protein post-translational modification process in PROTAC® technology, and is the key to the degradation of the protein of interest (POI). BOC Sciences has established an advanced one-stop Proteolysis Targeting Chimeric (PROTAC) service platform to provide customers with ubiquitylation assay services, and enzyme-linked immunosorbent assay (ELISA) as a traditional technical means can quantify the ubiquitylation level of proteins. BOC Sciences has always been committed to providing high-quality research outsourcing services and has extensive experience in ELISA technology.
Ubiquitylation refers to the process where ubiquitin specifically modifies the target protein under the action of a series of special enzymes. Ubiquitylation consists of three enzymatic reactions involving E1 ubiquitin activating enzymes, E2 ubiquitin conjugating enzymes, and E3 ubiquitin ligases. E1 ubiquitin activating enzymes activate ubiquitin and pass the activated ubiquitin to E2 ubiquitin conjugating enzymes. They promote the connection between their bound ubiquitin and POI through their active site cysteine. E3 ubiquitin ligases participate in ubiquitin transfer and promote the formation of β-peptide bonds between the target protein and the C-terminal carboxyl group of ubiquitin in the final stage. The protein substrate labeled with ubiquitin can be specifically recognized and degraded by the protease. Ubiquitinated proteins can be recognized by specific antibodies. ELISA is a qualitative and quantitative test of immune response using antibody-antigen specificity, and is a commonly used technical method in immunodiagnostic testing and biological science research. Anti-Ub antibody and ELISA technology can be used to quantitatively detect ubiquitinated protein, and then analyze the Ubiquitylation level of these proteins.
* PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.
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