Name: TL 13-27
Brand: BOC Sciences
Rating: 5 (1 reviews)

Purity

≥98%

ShelfLife

2 years

Storage

-20°C

Synonyms

N-(2-(2-(2-(4-(4-((5-Chloro-4-((2-(isopropylsulfonyl)phenyl)amino)pyrimidin-2-yl)amino)phenyl)piperazin-1-yl)ethoxy)ethoxy)ethyl)-2-((1,3-dioxo-2-(2-oxopiperidin-3-yl)isoindolin-4-yl)amino)acetamide

InChI Key

DOOUFCVVMSZNOA-UHFFFAOYSA-N

InChI

InChI=1S/C44H53ClN10O8S/c1-29(2)64(60,61)37-11-4-3-8-34(37)51-40-33(45)27-49-44(52-40)50-30-12-14-31(15-13-30)54-20-18-53(19-21-54)22-24-63-26-25-62-23-17-46-38(56)28-48-35-9-5-7-32-39(35)43(59)55(42(32)58)36-10-6-16-47-41(36)57/h3-5,7-9,11-15,27,29,36,48H,6,10,16-26,28H2,1-2H3,(H,46,56)(H,47,57)(H2,49,50,51,52)

SMILES

CC(C)S(=O)(=O)C1=CC=CC=C1NC2=NC(=NC=C2Cl)NC3=CC=C(C=C3)N4CCN(CC4)CCOCCOCCNC(=O)CNC5=CC=CC6=C5C(=O)N(C6=O)C7CCCNC7=O

Mechanism

Target: TL 13-27 is a negative control lacking productive kinase degradation activity.

Binding site: Its kinase-binding moiety can engage kinase ATP-binding regions without productive degradation.

Mechanism of action: TL 13-27 is an inactive negative control for the multitargeted kinase degrader TL 12-186. Although it retains a heterobifunctional PROTAC-like architecture, it does not induce detectable kinase degradation in vitro, making it useful for separating degradation-dependent effects from nonspecific compound exposure. In experimental workflows, TL 13-27 can control for target-ligand binding, linker-related physicochemical properties, CRBN ligand presentation, and cellular treatment conditions. It is particularly valuable in chemoproteomic degrader studies where interpretation requires distinguishing productive ubiquitin-proteasome engagement from simple kinase occupancy or off-target cellular stress.

Applications

• PROTAC-Mediated Targeted Degradation: TL 13-27 is designed to facilitate the targeted degradation of specific proteins by harnessing the ubiquitin-proteasome system. This application is crucial for investigating protein function and validating potential therapeutic targets in various cellular contexts.

• Protein Homeostasis Research: Utilizing TL 13-27 allows researchers to study the dynamics of protein homeostasis by selectively degrading proteins of interest. This aids in understanding the role of protein turnover in cellular processes and disease mechanisms.

• Functional Genomics Studies: TL 13-27 can be employed in functional genomics to achieve selective protein knockdown, providing insights into gene function and interactions. This application is vital for elucidating complex biological pathways and networks.

• Drug Discovery and Development: By using TL 13-27 in drug discovery, researchers can identify and validate novel drug targets through precise protein degradation. This approach accelerates the development of innovative therapeutic strategies by focusing on previously undruggable proteins.

1. Team-based learning: assessing the impact on anatomy teaching in People's Republic of China

Junhao Yan, Xinling Ding, Lili Xiong, E Liu, Yixuan Zhang, Yingjie Luan, Lihua Qin, Changman Zhou, Weiguang Zhang Adv Med Educ Pract. 2018 Aug 17;9:589-594.doi: 10.2147/AMEP.S169949.eCollection 2018.

Objectives:In this study, the effects of team-based learning (TBL) method on the anatomy course for students in People's Republic of China were assessed. Methods:The students were randomly divided into the traditional lecture-based teaching group (traditional learning [TL] group, 99 students) and TBL teaching group (98 students). The TBL method required the students to prepare the assigned content in advance and discuss some specific topics in small groups. The test scores and questionnaire were applied to evaluate the effects of the two methods. Results:The students in TBL group had higher examination scores (81.70±8.53 vs 74.41±8.27, F[1,195] =124.6, p<0.01). The ratios of students with excellent (13.27% vs 9.09%, χ2[1] =4.00, p=0.041) and good scores (25.51% vs 16.16%, χ2[1] =4.85, p=0.027) were markedly increased in the TBL than the TL group, and the ratio of students who had just managed to pass was decreased (17.34% vs 32.33% in TL group, χ2[1] =5.91, p=0.015). The students in TBL group significantly achieved some improvement in mutual communication ability (χ2[1] =7.54, p=0.006), expression ability (χ2[1] =4.930, p=0.026), generalization capacity (χ2[1] =4.08, p=0.043), cooperative ability cultivation (χ2[1] =5.04, p=0.024), knowledge extension (χ2[1] =4.50, p=0.034), and enthusiasm mobilization (χ2[1] =4.27, p=0.039).Conclusion:TBL could improve not only the test scores of the students, but also their study enthusiasm, initiative learning ability, communication ability, and team awareness.

2. Awareness of HCV Status and Preferences for Testing and Treatment among People with Recent Injecting Drug Use at a Peer-Led Needle and Syringe Program: The TEMPO Pilot Study

Anna Conway, Phillip Read, Rosie Gilliver, Tony McNaughton, Heather Valerio, Evan B Cunningham, Charles Henderson, Brett Hadlow, Katrina Molloy, Anna Doab, Shane Tillakeratne, Lucy Pepolim, Mary Ellen Harrod, Gregory J Dore, Jason Grebely Viruses. 2022 Nov 7;14(11):2463.doi: 10.3390/v14112463.

Background:New technologies and therapies allow the possibility of a single-visit test and treat model for hepatitis C virus (HCV), addressing some of the barriers to care faced by people who inject drugs.

3. Does providing real-time augmented feedback affect the performance of repeated lower limb loading to exhaustion?

Amitabh Gupta, Ryan J Hilliard, Kurt L Mudie, Peter J Clothier Gait Posture. 2016 Feb;44:204-8.doi: 10.1016/j.gaitpost.2015.12.034.Epub 2015 Dec 24.

Introduction:This study aimed to determine whether real-time augmented feedback influenced performance of single-leg hopping to volitional exhaustion. Methods:Twenty-seven healthy, male participants performed single-leg hopping (2.2 Hz) with (visual and tactile feedback for a target hop height) or without feedback on a force plate. Repeated measures ANOVA were used to determine differences in vertical stiffness (k), duration of flight (tf) and loading (tl) and vertical height displacement during flight (zf) and loading (zl). A Friedman 2-way ANOVA was performed to compare the percentage of trials between conditions that were maintained at 2.2 Hz ± 5%. Correlations were performed to determine if the effects were similar when providing tactile or visual feedback synchronously with the audible cue. Results:Augmented feedback resulted in maintenance of the tf, zf and zl between the start and end of the trials compared to hopping with no feedback (p70% of total hops at 2.2 ± 5% Hz and this was significantly lower (p=0.01) with tactile (13/27) and visual (15/27) feedback. There was a strong correlation between tactile and visual feedback for duration of hopping cycle (Spearman's r=0.74, p ≤ 0.01).Conclusion:Feedback was detrimental to being able to maintain hopping cadence in some participants while other participants were able to achieve the cadence and target hop height. This indicates variability in the ability to use real-time augmented feedback effectively.

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Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C₁V₁ = C₂V₂