Pomalidomide-PEG4-Alkyne

Background Introduction

Pomalidomide-PEG4-Alkyne is a specialized PROTAC building block featuring the E3 ligase ligand pomalidomide, a polyethylene glycol (PEG4) flexible linker, and a terminal alkyne reactive group. This unique conjugate is designed for the rapid and modular assembly of PROTACs and related bifunctional molecules that harness targeted protein degradation via the ubiquitin-proteasome system.

Mechanism

The mechanism of Pomalidomide-PEG4-Alkyne involves targeted recruitment of the E3 ubiquitin ligase cereblon (CRBN) through its pomalidomide moiety. The PEG4 linker provides molecular flexibility, allowing optimal spatial arrangement between the ligase and a target protein binder. The terminal alkyne group enables efficient conjugation with azide-functionalized molecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC or ‘click’ chemistry), facilitating the creation of diverse, high-purity bifunctional degraders that induce selective ubiquitination and proteasomal degradation of target proteins.

Applications

Pomalidomide-PEG4-Alkyne is widely applied in the design and synthesis of next-generation PROTACs, molecular glues, and other targeted protein degradation tools. Researchers use this conjugate to connect pomalidomide to a variety of target protein ligands, expanding the chemical diversity and functional capabilities of custom degrader molecules. Its utility extends to drug discovery, chemical biology studies, and validation of novel protein targets using targeted protein degradation as a mechanism of action.

• Alkyne-functionalized PEG4 linker enables versatile bioconjugation via click chemistry for efficient PROTAC assembly.
• Pomalidomide moiety ensures high affinity recruitment of CRBN E3 ligase, optimizing targeted protein degradation.