ALV2

Background Introduction

ALV2 is a potent and selective small molecule ligand designed to bind the Von Hippel-Lindau (VHL) E3 ubiquitin ligase. VHL ligands are essential components in the PROTAC (Proteolysis Targeting Chimera) technology landscape, facilitating targeted protein degradation by leveraging the endogenous ubiquitin-proteasome system. ALV2 features an optimized structure that offers improved binding affinity and synthetic adaptability, making it a preferred choice for constructing next-generation VHL-based PROTACs.

Mechanism

ALV2 works by specifically engaging the VHL E3 ligase, enabling the recruitment of the CUL2-VHL E3 ubiquitin ligase complex. When appended to a target protein ligand via a suitable linker, ALV2 forms the E3 ligase recruitment module of a PROTAC molecule. This bifunctional chimera brings the target protein into close proximity with the VHL ligase complex, triggering polyubiquitination and subsequent proteasomal degradation of the protein of interest. The rationally engineered features of ALV2 provide expanded linker design and attachment options, supporting efficient PROTAC construction and enhanced degradation activity.

Applications

ALV2 is widely used in the development of VHL-recruiting PROTACs for preclinical and clinical research applications such as:

• Synthesis of bispecific protein degraders targeting disease-relevant proteins (kinases, transcription factors, etc.)
• Structure-activity relationship (SAR) studies to optimize degrader performance
• Creation of molecular probes to study protein function via selective degradation
• Early-stage drug discovery, target validation, and mechanism-of-action studies
• Customizable platforms for academic and pharmaceutical research into targeted protein degradation

• High-purity compound verified by HPLC, NMR, and LC-MS
• Consistent batch-to-batch reproducibility with complete QC documentation
• Supplied with COA, MSDS, and analytical data for traceability
• Reliable global shipping with stability-guaranteed packaging
• Dedicated technical support and optional custom synthesis service
• Demonstrates strong binding affinity to CRBN, VHL, or other E3 ligases
• Enables stable E3 ligase recruitment for targeted protein degradation
• Optimized for high-affinity binding to VHL E3 ligase, enhancing protein degradation efficiency in PROTAC applications
• Excellent chemical stability and solubility, facilitating reliable linker and payload conjugation during synthesis