Name: Biotin-PEG4-NHS ester
Brand: BOC Sciences
Rating: 5 (1 reviews)

Size

Price

Stock

Quantity

$--

In stock

Purity

>97%

Solubility

In DMSO: 125 mg/mL (212.34 mM; Need ultrasonic)

Appearance

Solid powder

Storage

-20°C, protect from light. The solution be prepared immediately before its experimental application.

Shipping

Room temperature in continental US; may vary elsewhere.

IUPACName

(2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]ethoxy]propanoate

Synonyms

3-[2-[2-[2-[2-[[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]ethoxy]ethoxy]ethoxy]ethoxy]propanoic acid (2,5-dioxo-1-pyrrolidinyl) ester; (2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]ethoxy]propanoate

Density

1.3±0.1 g/cm3

InChI Key

DTLVBHCSSNJCMJ-JXQFQVJHSA-N

InChI

InChI=1S/C25H40N4O10S/c30-20(4-2-1-3-19-24-18(17-40-19)27-25(34)28-24)26-8-10-36-12-14-38-16-15-37-13-11-35-9-7-23(33)39-29-21(31)5-6-22(29)32/h18-19,24H,1-17H2,(H,26,30)(H2,27,28,34)/t18-,19-,24-/m0/s1

SMILES

C1CC(=O)N(C1=O)OC(=O)CCOCCOCCOCCOCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3

1. Catalytic antibodies

A Tramontano, R A Lerner, K D Janda Science . 1986 Dec 19;234(4783):1566-70. doi: 10.1126/science.3787261.

Monoclonal antibodies elicited to haptens that are analogs of the transition state for hydrolysis of carboxylic esters behaved as enzymic catalysts with the appropriate substrates. These substrates are distinguished by the structural congruence of both hydrolysis products with haptenic fragments. The haptens were potent inhibitors of this esterolytic activity, in agreement with their classification as transition state analogs. Mechanisms are proposed to account for the different chemical behavior of these antibodies with two types of ester substrates. The generation of an artificial enzyme through transition state stabilization by antibodies was thus demonstrated. These studies indicate a potentially general approach to catalyst design.

2. Lactose esters: synthesis and biotechnological applications

Maciej Guzik, Jakub Staroń, Janusz M Dąbrowski, Ewelina Cichoń Crit Rev Biotechnol . 2018 Mar;38(2):245-258. doi: 10.1080/07388551.2017.1332571.

Biodegradable nonionic sugar esters-based surfactants have been gaining more and more attention in recent years due to their chemical plasticity that enables the various applications of these molecules. In this review, various synthesis methods and biotechnological implications of lactose esters (LEs) uses are considered. Several chemical and enzymatic approaches are described for the synthesis of LEs, together with their applications, i.e. function in detergents formulation and as additives that not only stabilize food products but also protect food from undesired microbial contamination. Further, this article discusses medical applications of LEs in cancer treatment, especially their uses as biosensors, halogenated anticancer drugs, and photosensitizing agents for photodynamic therapy of cancer and photodynamic inactivation of microorganisms.

3. Palladium-Catalyzed Tandem Ester Dance/Decarbonylative Coupling Reactions

Eisuke Ota, Naomi Inayama, Junichiro Yamaguchi, Masayuki Kubo Org Lett . 2022 Jun 3;24(21):3855-3860. doi: 10.1021/acs.orglett.2c01432.

"Dance reaction" on the aromatic ring is a powerful method in organic chemistry to translocate functional groups on arene scaffolds. Notably, dance reactions of halides and pseudohalides offer a unique platform for the divergent synthesis of substituted (hetero)aromatic compounds when combined with transition-metal-catalyzed coupling reactions. Herein, we report a tandem reaction of ester dance and decarbonylative coupling enabled by palladium catalysis. In this reaction, 1,2-translocation of the ester moiety on the aromatic ring is followed by decarbonylative coupling with nucleophiles to enable the installation of a variety of nucleophiles at the position adjacent to the ester in the starting material.

ConcentrationVolumeMass	1 mg	5 mg	10 mg
1 mM	1.6987 mL	8.4937 mL	16.9874 mL
5 mM	0.3397 mL	1.6987 mL	3.3975 mL
10 mM	0.1699 mL	0.8494 mL	1.6987 mL

Biotin-PEG4-NHS ester is a PEG-based biotinylation linker designed for efficient conjugation of biomolecules in PROTAC workflows and related targeted degradation research. Its NHS ester enables rapid acylation of primary amines, while the PEG spacer improves aqueous solubility and reduces steric constraints, supporting stable, functional conjugate formation. The following sections describe the linker’s structural features and practical reactivity considerations in detail below.

Structure: The linker comprises a biotin moiety connected through a polyethylene glycol spacer to an N-hydroxysuccinimide ester. It contains an NHS-activated carboxylate, ether linkages within the PEG chain, and amide-forming functionality upon reaction. Overall, it is engineered for water-compatible, flexible spacing and stable conjugate generation.

Reactivity: The NHS ester is typically used to form amide bonds with primary amines on proteins, peptides, or amine-functionalized PROTAC components. Conjugations are commonly performed under mildly basic aqueous conditions to promote nucleophilic acyl substitution, with careful control of pH and NHS hydrolysis. Suitable solvents include buffered aqueous media; catalysts are generally not required. After coupling, excess activated ester is removed by standard purification to obtain defined biotinylated conjugates for downstream targeted degradation experiments.

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Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C₁V₁ = C₂V₂