1.[Study on main pharmacodynamic effects for Schisandra lignans based upon network pharmacology].
Qiu HT, Zhao XP, Li Z, Wang LL, Wang Y. Zhongguo Zhong Yao Za Zhi. 2015 Feb;40(3):522-7.
In this study, we focused on the study of pharmacodynamic effects for 6 major bioactive lignans of Schisandra chinensis, namely deoxyschizandrin, schisandrin B, schisandrin C, schisandrin, schizandrol B and schisantherin A. A compound-gene-pathway network, which contained 124 related genes and 88 pathways, was constructed by collecting drug genes through mining relevant literatures and network pharmacology analysis. Based on the network analysis, 32 pathways and 80 related genes were associated with inflammation, which implied that anti-inflammatory might be the major pharmacodynamic effects of these compounds. All lignans except schizandrol B reduced LPS-induced NO production in RAW264.7 cells, which validated the anti-inflammatory hypothesis generated from network analysis. Furthermore, we investigated the effects of deoxyschizandrin, schisandrin C, schisandrin and schisantherin A on the secretion of inflammatory cytokines TNF-α, IL-1β, IL-6, PGE2 and protein expressions of iNOS, COX-2.
2.Smashing Tissue Extraction of Five Lignans From the Fruit of Schisandra chinensis.
Cheng Z1, Song H2, Yang Y3, Zhou H1, Liu Y1, Liu Z1. J Chromatogr Sci. 2016 Feb;54(2):246-56. doi: 10.1093/chromsci/bmv116. Epub 2015 Aug 8.
Schisandra chinensis is one of the most famous herbal medicines in China, Korea and Japan. It has been widely used as a tonic, sedative, anti-aging and astringent agent. Lignans are one of its main bioactive components. The classical methods for extracting lignans, however, were tedious and energy-consuming. With the aim to develop an effective extraction method of lignans, the smashing tissue extraction (STE) technique was adopted and optimized in this study. Extraction conditions of STE have been optimized by the response surface methodology based on the Box-Behnken design. Results showed that 75% aqueous ethanol was the optimal extraction solvent, and the other optimal conditions were as follows: extraction voltage of 180 V, extraction time of 1 min, solid-liquid ratio of 1 : 19 and sample particle size of 120 mesh. Under these optimized conditions, the total content of the five lignans (Schisandrol A, Schisantherin A, Deoxyschisandrin, Schisandrin B and Schisandrin C) in S.
3.[Quality characteristic comparison of Schisandrae Chinensis Fructus from different place].
Zhou YF, Wang JB, Zhang DK, Tan P, Zhang HZ, Li BC, Xiao XH. Zhongguo Zhong Yao Za Zhi. 2015 Aug;40(16):3152-7.
The contents of schisandrol A, schisandrol B, schisantherin A, schisandrin A , schisandrin B, schisandrin C in Schisandrae Chinensis Fructus (SCF) were determined simultaneously by HPLC. Collect 100-seed weight, color, pulp content, longitude and latitude of SCF of different batches were collected. SIMCA-P and SPSS were applied to make PLS-DA analysis of 24 batches of SCF and correlation analysis of relevant parameters. According to the 13 parameters, SCF from three different places of origin could be distinguished effectively. It was found that the content of chemical component of SCF increased with latitude and longitude first, and then decrease. The results provide some theoretical basis for study of SCF genuineness and traditional method of identifying just from experience.
4.[Study on chemical constituents from Schisandra chinensis stem].
Zheng LS, Du SS, Cai Q. Zhong Yao Cai. 2014 Oct;37(10):1803-6.
OBJECTIVE: To separate and identify the chemical constituents from the stem of Schisandra chinensis.
