DBCO-NHCO-PEG2-amine
Cat No.: BP-502119
4.5 
DBCO-NHCO-PEG2-amine is a heterobifunctional PEG-based linker featuring a dibenzocyclooctyne (DBCO) moiety for strain-promoted azide–alkyne cycloaddition (SPAAC) and a terminal amide/amine functionality for further conjugation. Structurally, it comprises a short polyethylene glycol segment that provides hydrophilicity and conformational flexibility, while the DBCO group enables rapid, catalyst-free coupling to azide-bearing partners under mild conditions. In PROTAC and targeted degradation workflows, this linker is valuable for modular assembly: it can be used to attach an azide-functional recruiting ligand, warhead, or solubilizing handle to a complementary component bearing the DBCO group, forming a stable triazole linkage without copper contamination that could otherwise affect sensitive biomolecules. The PEG spacer helps reduce steric hindrance at the conjugation junction and can improve aqueous handling, thereby facilitating systematic optimization of degrader constructs for mechanistic and structure–function studies.
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* For research and manufacturing use only. Not for human or clinical use.
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This PROTAC linker, DBCO-NHCO-PEG2-amine, is designed to connect targeting ligands to azide-bearing partners through strain-promoted cycloaddition, enabling efficient conjugation under biocompatible conditions. Its PEG-based spacer and amide-bearing handle support controlled linker flexibility and stable amide linkage formation, which can improve the spatial presentation of recruited proteins in targeted degradation workflows. The detailed structural and reactivity characteristics are described below.
Structure: The linker contains a dibenzocyclooctyne (DBCO) moiety for strain-promoted alkyne–azide cycloaddition, an amide (NHCO) connecting functional segments, and a short polyethylene glycol (PEG) spacer terminating in a primary amine. Its conjugation-ready functional groups support stable covalent attachment and increased hydrophilicity.
Reactivity: DBCO reacts selectively with azides via strain-promoted cycloaddition, typically proceeding without added copper catalysts, which is advantageous for sensitive ligands and protein-conjugation workflows. The amine can be used for standard amide/urea coupling or for introducing the linker into broader PROTAC architectures using activated carboxylic acids or suitable electrophiles. Reactions are commonly performed in polar organic or mixed aqueous buffers compatible with the azide partner and maintaining ligand integrity.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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