m-PEG13-NHS ester
Cat No.: BP-501857
4.5 
m-PEG13-NHS ester is a methoxy-terminated polyethylene glycol linker bearing an N-hydroxysuccinimide (NHS) ester at one end, providing a watersoluble, flexible spacer of intermediate chain length. The PEG segment confers reduced nonspecific interactions and improved biocompatible handling, while the NHS ester enables efficient, chemoselective coupling to primary amines on lysine residues of proteins or on amino-functional small molecules, forming stable amide bonds under standard aqueous buffer conditions. In PROTAC design, this linker is used to connect or spatially separate the ligand-binding modules so that the recruited E3 ligase and target-binding moieties can adopt productive geometries for ternary complex formation. Its modular reactivity and solubility make it valuable for constructing multicomponent targeted degradation reagents, facilitating systematic optimization of linker length and attachment site to improve degradation potency and experimental robustness.
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* For research and manufacturing use only. Not for human or clinical use.
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m-PEG13-NHS ester is a polyethylene glycol (PEG)-based N-hydroxysuccinimide (NHS) ester linker designed for efficient conjugation in PROTAC workflows. Its PEG segment provides aqueous solubility and conformational flexibility, while the NHS ester enables fast formation of stable amide bonds with primary amine-bearing ligands. This combination supports reliable assembly of bifunctional degraders, and the detailed structural and reactivity considerations are provided below.
Structure: The linker comprises an m-PEG chain terminated by an NHS ester, featuring an activated carboxylate adjacent to a succinimide leaving group. It is characterized by ether-rich PEG connectivity, an amide-forming acyl-activated motif, and flexible, hydrophilic segments that enhance water compatibility.
Reactivity: NHS esters react with primary amines via nucleophilic acyl substitution, yielding amide linkages while releasing NHS. Suitable conditions typically use mildly basic aqueous or mixed aqueous/organic buffers that maintain NHS ester stability, with careful control of pH to balance activation and minimize hydrolysis. Common solvents include water and alcohol-containing media; no special catalysts are required, but inert handling and timely coupling are recommended to limit side reactions from hydrolysis.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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