N-(Azido-PEG2)-N-Boc-PEG4-NHS ester
CAS No.: 2093153-95-6
Cat No.: BP-500903
Purity: 98%
4.5 
N-(Azido-PEG2)-N-Boc-PEG4-NHS ester is a multifunctional, heterobifunctional PEG-based PROTAC linker designed for controlled conjugation chemistry. Structurally, it contains an NHS ester for efficient reaction with primary amines on target-binding ligands, a protected amine (Boc) that can be deprotected to enable subsequent coupling steps, and a terminal azide handle for orthogonal bioorthogonal labeling or click-mediated attachment to a second component. The PEG segments provide aqueous solubility and conformational flexibility, helping to reduce steric interference and to tune the effective distance between the ligands that recruit an E3 ligase and the target protein. In targeted protein degradation research, this linker enables modular assembly of PROTACs by first installing the NHS-activated portion onto an amine-functionalized ligand, then using the azide to connect the remaining module under conditions compatible with sensitive biomolecules. Its orthogonality and tunable spacing make it valuable for systematic optimization of degrader architectures and for generating fluorescent or affinity-tagged intermediates.
Structure of 2093153-95-6
Assurance
Delivery
Support
* For research and manufacturing use only. Not for human or clinical use.
| Size | Price | Stock | Quantity |
|---|---|---|---|
| -- | $-- | In stock |
Looking for different specifications? Click to request a custom quote!
Capabilities & Facilities
- Comprehensive PROTAC Platform
- Scientific Expertise & Technical Support
- Custom Synthesis & Design Service
- Extensive Product Coverage
- Cutting-Edge Innovation
- Fast Delivery & Global Support
- 24/7 customer service
- 100% quality assurance
Popular Publications Citing BOC Sciences Products
N-(Azido-PEG2)-N-Boc-PEG4-NHS ester, is designed to enable modular assembly of targeted protein degraders by combining an NHS-ester handle for efficient conjugation with an azide-bearing PEG segment for subsequent bioorthogonal coupling. Its PEG-rich architecture supports aqueous solubility and flexible spacer behavior, which can improve linker presentation and conjugate accessibility in PROTAC constructs. The details below describe its structure and the practical reactivity profile for PROTAC synthesis.
Structure: The molecule contains an NHS ester for acyl transfer, a Boc-protected amine for controlled deprotection, and a terminal azide for click-type chemistry. Multiple ethylene glycol units provide a hydrophilic, flexible PEG spacer. It features amide and carbamate linkages, with an overall amphiphilic character suitable for aqueous conjugation workflows.
Reactivity: The NHS ester reacts with primary amines under mildly basic aqueous or mixed solvent conditions to form stable amide bonds, enabling attachment to lysine-containing ligands or engineered amine-bearing targeting modules. The azide group is compatible with strain-promoted or Cu(I)-catalyzed azide–alkyne cycloaddition for orthogonal installation of the complementary partner. Boc can be removed using standard acid-mediated deprotection when required for further coupling, while the azide remains stable under typical NHS-ester conjugation conditions.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
Related Product Recommendations
Please contact us with any specific requirements and we will get back to you as soon as possible.
