N-Hydroxysulfosuccinimidyl-4-azido benzoate
Cat No.: BP-502172
4.5 
N-Hydroxysulfosuccinimidyl-4-azido benzoate is an azido-functional, sulfonated N-hydroxysulfosuccinimide (sulfo-NHS) ester designed for efficient, aqueous-compatible amide coupling. Structurally, it contains a reactive sulfo-NHS ester that acylates primary amines on proteins or linkers, together with a para-azidobenzoate handle that can be retained for subsequent bioorthogonal conjugation. In PROTAC construction, this reagent serves as a modular coupling intermediate: it first installs the 4-azidobenzoate moiety onto an amine-bearing targeting ligand or scaffold, enabling controlled attachment while maintaining the azide for later “click” reactions (e.g., strain-promoted azide–alkyne cycloaddition) to connect to a second component such as a cereblon- or VHL-binding element. Its value for targeted protein degradation research lies in providing a robust, water-friendly strategy to generate well-defined, multifunctional PROTAC precursors with orthogonal reactivity for stepwise synthesis and purification.
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* For research and manufacturing use only. Not for human or clinical use.
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N-Hydroxysulfosuccinimidyl-4-azido benzoate is a water-soluble, aryl azide–containing PROTAC linker reagent designed to enable efficient conjugation between targeting ligands and subsequent functionalization steps. Its activated N-hydroxysulfosuccinimide ester supports robust amide bond formation under mild conditions, while the azido group provides a versatile handle for bioorthogonal or click-type coupling strategies used in targeted protein degradation workflows. The points below describe its structure and practical reactivity for PROTAC assembly in detail.
Structure: The molecule combines a sulfonated N-hydroxysuccinimide ester with a para-substituted azido benzoate core. It contains an aryl azide moiety, an activated ester functional group, and multiple heteroatoms that confer aqueous solubility. The presence of ester and azide functionalities enables sequential conjugation and downstream coupling.
Reactivity: The N-hydroxysulfosuccinimide ester reacts with primary amines to form stable amide linkages via nucleophilic acyl substitution, typically using buffered aqueous or mixed solvent systems at conditions compatible with amine-bearing ligands. The azido group is retained during ester coupling and can later participate in bioorthogonal ligation approaches under appropriate activation and catalyst choices. Common bases and coupling buffers are selected to preserve azide integrity while maximizing amide formation efficiency.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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