Pomalidomide-d5

Background Introduction

Pomalidomide-d5 is the deuterated form of pomalidomide, a well-characterized immunomodulatory imide drug (IMiD) that serves as a potent ligand for the Cereblon (CRBN) E3 ubiquitin ligase. This stable isotope-labeled analog contains five deuterium atoms, making it a unique tool for pharmacokinetic studies and tracer experiments as well as an effective building block for proteolysis targeting chimera (PROTAC) design. Leveraging the biological activity of pomalidomide, pomalidomide-d5 supports quantitative analysis and advanced research in targeted protein degradation (TPD) technologies.

Mechanism

Pomalidomide-d5 binds selectively to the CRBN component within the CUL4-CRBN E3 ubiquitin ligase complex. Through this CRBN recognition, it mediates the recruitment of the ligase machinery to the protein of interest when incorporated into a PROTAC construct. This recruitment enables targeted ubiquitination and subsequent degradation of the target protein via the proteasomal pathway. The incorporation of deuterium atoms provides enhanced analytical capabilities for mass spectrometry, offering increased stability and facilitating differentiation in metabolic or in vivo studies compared to non-deuterated forms.

Applications

Pomalidomide-d5 serves as a crucial intermediate in the synthesis of CRBN-recruiting PROTACs and molecular glues. Its deuterated nature is especially valuable for ADME (absorption, distribution, metabolism, and excretion) studies, bioanalytical quantification, and tracking the fate of PROTACs in biological systems. Key applications include:

• Synthesis of CRBN-based PROTACs for targeted protein degradation research
• Use as an internal standard or tracer in LC-MS/MS studies for pharmacokinetic and metabolomic analysis
• Evaluation of metabolic stability and isotope effect studies on degrader molecules
• SAR (structure-activity relationship) and medicinal chemistry optimization projects
• Support for CRO services and academic research requiring stable isotope-labeled E3 ligase ligands

• High-purity compound verified by HPLC, NMR, and LC-MS
• Consistent batch-to-batch reproducibility with complete QC documentation
• Supplied with COA, MSDS, and analytical data for traceability
• Reliable global shipping with stability-guaranteed packaging
• Dedicated technical support and optional custom synthesis service
• Demonstrates strong binding affinity to CRBN, VHL, or other E3 ligases
• Enables stable E3 ligase recruitment for targeted protein degradation
• Deuterium labeling enables precise quantitative analysis in PROTAC research and mass spectrometry applications.
• High purity and structural fidelity make it ideal for synthesizing stable CRBN E3 ligase recruiting elements.