Name: Bis-PEG6-NHS ester
Brand: BOC Sciences
Rating: 5 (1 reviews)

Size

Price

Stock

Quantity

$--

In stock

Purity

≥95%

Solubility

Soluble in DCM, DMSO

Appearance

Pale Yellow or Colorless Oily Matter

ShelfLife

-20°C 3 years powder; -80°C 2 years in solvent

Storage

Store at 2-8°C

Shipping

Room temperature

IUPACName

(2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[2-[2-[2-[3-(2,5-dioxopyrrolidin-1-yl)oxy-3-oxopropoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate

Synonyms

NHS-PEG6-NHS; 4,7,10,13,16,19-Hexaoxadocosanedioic acid, 1,22-bis(2,5-dioxo-1-pyrrolidinyl) ester; Bis(2,5-dioxopyrrolidin-1-yl) 4,7,10,13,16,19-hexaoxadocosanedioate; Di-NHS Ester-PEG6; 1,1'-[(1,22-Dioxo-4,7,10,13,16,19-hexaoxadocosane-1,22-diyl)bis(oxy)]di(2,5-pyrrolidinedione); 2,5-Pyrrolidinedione, 1,1'-[(1,22-dioxo-4,7,10,13,16,19-hexaoxadocosane-1,22-diyl)bis(oxy)]bis-

Boiling Point

659.4±65.0°C at 760 mmHg

Density

1.4±0.1 g/cm3

InChI Key

FDLGRFGWAMAWTE-UHFFFAOYSA-N

InChI

InChI=1S/C24H36N2O14/c27-19-1-2-20(28)25(19)39-23(31)5-7-33-9-11-35-13-15-37-17-18-38-16-14-36-12-10-34-8-6-24(32)40-26-21(29)3-4-22(26)30/h1-18H2

SMILES

C1CC(=O)N(C1=O)OC(=O)CCOCCOCCOCCOCCOCCOCCC(=O)ON2C(=O)CCC2=O

Biological Activity

Bis-PEG6-NHS ester is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs)[1] . In Vitro: ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.

ConcentrationVolumeMass	1 mg	5 mg	10 mg
1 mM	1.7345 mL	8.6723 mL	17.3445 mL
5 mM	0.3469 mL	1.7345 mL	3.4689 mL
10 mM	0.1734 mL	0.8672 mL	1.7345 mL

Bis-PEG6-NHS ester, is a bifunctional polyethylene glycol (PEG)-based PROTAC linker designed to enable efficient conjugation of two ligands through N-hydroxysuccinimide (NHS) ester chemistry. Its PEG spacer improves solubility and provides conformational flexibility, supporting productive ternary complex formation in targeted protein degradation workflows. Detailed structural and reactivity considerations are provided below to guide experimental PROTAC construction.

Structure: Bis-PEG6-NHS ester contains a PEG-based, flexible spacer terminated with NHS ester groups. The NHS esters are activated carboxylate derivatives, enabling acyl transfer to nucleophiles. The molecule features ether linkages within the PEG chain and amide-forming potential upon reaction, yielding stable conjugates.

Reactivity: Suitable reactions typically use amine-containing ligands or amino-functionalized targeting moieties to drive NHS ester acylation. The process proceeds via nucleophilic attack on the activated carbonyl, forming an amide bond with concomitant NHS release. Common conditions employ aqueous or mixed aqueous buffers at mildly basic pH, with NHS esters handled under controlled moisture exposure. No specialized catalysts are required; solvent choice balances solubility and nucleophile availability.

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* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C₁V₁ = C₂V₂