Chloroacetamido-C4-NHBoc
CAS No.: 133264-58-1
Cat No.: BP-500740
4.5 
Chloroacetamido-C4-NHBoc is a bifunctional PROTAC linker building block featuring a chloroacetamide electrophile and a protected amine (Boc) separated by a four-carbon (C4) spacer. The chloroacetamide moiety is designed to undergo chemoselective nucleophilic substitution with thiol- or amine-containing handles on ligands or intermediate fragments, enabling stable covalent conjugation under standard PROTAC synthesis conditions. The C4 chain provides a defined linker length and conformational flexibility that can improve productive spatial alignment between the two binding modules of a PROTAC, supporting efficient formation of a ternary complex with the target protein and the E3 ligase. The Boc-protected amine serves as a controllable point for subsequent coupling steps after deprotection. This linker is valuable for constructing modular targeted protein degradation reagents where reproducible attachment chemistry and tunable geometry are critical for optimizing degradation potency and selectivity.
Structure of 133264-58-1
Assurance
Delivery
Support
* For research and manufacturing use only. Not for human or clinical use.
| Size | Price | Stock | Quantity |
|---|---|---|---|
| -- | $-- | In stock |
Looking for different specifications? Click to request a custom quote!
Capabilities & Facilities
- Comprehensive PROTAC Platform
- Scientific Expertise & Technical Support
- Custom Synthesis & Design Service
- Extensive Product Coverage
- Cutting-Edge Innovation
- Fast Delivery & Global Support
- 24/7 customer service
- 100% quality assurance
Popular Publications Citing BOC Sciences Products
This chloroacetamide-based C4 linker is designed for efficient installation of amine-bearing warheads in targeted protein degradation (PROTAC) workflows. Its electrophilic chloroacetamide functionality enables reliable coupling to nucleophilic groups, supporting modular PROTAC synthesis and subsequent optimization of linker length and attachment geometry. The features and practical considerations for assembling PROTAC constructs using this linker are described in detail below.
Structure: The linker contains a chloroacetamide electrophile and a protected amine (Boc) that supports controlled reactivity. It features amide and carbamate functionalities, along with a flexible aliphatic C4 spacer that can tune conformational freedom and attachment distance in PROTAC architectures.
Reactivity: The chloroacetamide moiety is suitable for nucleophilic substitution by amines under standard organic synthesis conditions, forming a stable thioether-free amide-linked junction typical of PROTAC intermediate construction. Use compatible polar aprotic or mixed solvents, and maintain conditions that preserve the Boc protecting group until intentional deprotection. No special catalysts are generally required for the substitution step, but base selection should match the nucleophile strength and avoid side reactions.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
Related Product Recommendations
Please contact us with any specific requirements and we will get back to you as soon as possible.
