Fmoc-NH-PEG12-NHS ester
CAS No.: 2227246-92-4
Cat No.: BP-501103
Purity: ≥95%
4.5 
Fmoc-NH-PEG12-NHS ester is a heterobifunctional PEG-based linker designed for PROTAC and targeted protein degradation workflows that require a stable amide-forming handle and controlled spatial separation. Structurally, it combines an Fmoc-protected amine for orthogonal solid-phase or surface coupling with a terminal N-hydroxysuccinimide (NHS) ester that reacts efficiently with primary amines on lysine residues or engineered protein tags to form robust amide bonds. The PEG12 chain provides a hydrophilic, flexible spacer that can reduce steric interference and help preserve the binding conformations of the two PROTAC-recruiting ligands, thereby improving productive ternary complex formation. In experimental PROTAC design, this linker is valuable for conjugating targeting and E3 ligase-binding moieties to generate degraders with tunable linker length and attachment chemistry, facilitating systematic optimization of degradation potency and selectivity in biochemical and cellular assays.
Structure of 2227246-92-4
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Fmoc-NH-PEG12-NHS ester is a PEG-based bifunctional linker designed for efficient conjugation in PROTAC assembly workflows. The NHS ester enables rapid formation of stable amide bonds with primary amines, while the Fmoc group provides orthogonal protection and compatibility with stepwise synthesis. This combination supports modular construction of targeted protein degraders with controlled linker length and improved solubility. Detailed structural and reactivity considerations are provided below.
Structure: The molecule contains an Fmoc-protected amine linked to a polyethylene glycol chain terminated by an N-hydroxysuccinimide ester. It features an NHS-activated carboxylate, aromatic Fmoc functionality, ether-rich PEG segments, and amide-forming reactive carbonyl chemistry, yielding a polar, water-compatible linker.
Reactivity: The NHS ester reacts with primary amines under mild aqueous or mixed aqueous conditions to form amide bonds via nucleophilic acyl substitution, typically favoring near-neutral pH to maintain amine nucleophilicity while limiting hydrolysis. Common bases are used sparingly to balance reactivity and stability. After coupling, the Fmoc group can be removed under standard deprotection conditions to enable subsequent functionalization steps in PROTAC synthesis.
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Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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