DBCO-C-PEG1

This DBCO-C-PEG1 linker is designed for efficient conjugation in PROTAC workflows that require robust, bioorthogonal attachment handles. The combination of a DBCO click moiety with a short polyethylene glycol segment supports reliable coupling to targeting ligands and facilitates the construction of degraders with improved conjugation flexibility. The linker’s use in PROTAC technology enables modular assembly under conditions compatible with sensitive biomolecule conjugates;

Structure: The linker contains a DBCO (dibenzocyclooctyne) cyclooctyne core for strain-promoted cycloaddition, connected to a short PEG chain that provides hydrophilicity and conformational flexibility. It features aromatic rings, ether linkages within the PEG segment, and a strained alkyne suitable for rapid bioorthogonal reactions.

Reactivity: DBCO-C-PEG1 participates in strain-promoted azide–alkyne cycloaddition with azide-bearing partners, enabling catalyst-free conjugation. Typical conditions use aqueous or mixed aqueous buffers at neutral pH, with mild temperatures to preserve ligand integrity. The mechanism proceeds via a cycloaddition between the strained cyclooctyne and an azide, forming a stable triazole linkage; no copper catalyst is required, which helps minimize side reactions during PROTAC assembly.