Name: Mal-PEG5-NHS ester
Brand: BOC Sciences
Rating: 5 (1 reviews)

Size

Price

Stock

Quantity

$--

In stock

Purity

≥95%

Solubility

Soluble in DCM, DMF, DMSO

Appearance

Pale Yellow or Colorless Oily Matter

Storage

Store at 2-8°C

Shipping

Room temperature in continental US; may vary elsewhere.

IUPACName

(2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[2-[2-[2-(2,5-dioxopyrrol-1-yl)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate

Synonyms

Mal-PEG5-NHS; 2,5-dioxopyrrolidin-1-yl 1-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-3,6,9,12,15-pentaoxaoctadecan-18-oate; 1-{18-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-18-oxo-3,6,9,12,15-pentaoxaoctadec-1-yl}-1H-pyrrole-2,5-dione; 1H-Pyrrole-2,5-dione, 1-[18-[(2,5-dioxo-1-pyrrolidinyl)oxy]-18-oxo-3,6,9,12,15-pentaoxaoctadec-1-yl]-

Boiling Point

608.4±65.0°C at 760 mmHg

Density

1.3±0.1 g/cm3

InChI Key

PDFXZGGPCFZXJU-UHFFFAOYSA-N

InChI

InChI=1S/C21H30N2O11/c24-17-1-2-18(25)22(17)6-8-30-10-12-32-14-16-33-15-13-31-11-9-29-7-5-21(28)34-23-19(26)3-4-20(23)27/h1-2H,3-16H2

SMILES

C1CC(=O)N(C1=O)OC(=O)CCOCCOCCOCCOCCOCCN2C(=O)C=CC2=O

Mal-PEG5-NHS ester is a heterobifunctional PEG-based linker designed for efficient conjugation in targeted protein degradation workflows. Its NHS-ester handle enables rapid amide-bond formation with primary amines on ligands or protein-binding modules, while the maleimide functionality supports selective coupling to thiols. This combination supports modular PROTAC assembly with improved solubility and tunable linker length, and

Structure: The molecule contains a maleimide group for thiol-reactive Michael-type addition and an N-hydroxysuccinimide ester for amine acylation. A PEG segment provides flexible, hydrophilic spacing. The linker features an activated carboxylate (NHS ester) and conjugated alkene within the maleimide ring, improving aqueous compatibility.

Reactivity: For PROTAC construction, the NHS ester is typically reacted with primary amines under mildly basic aqueous conditions to form stable amide bonds, after which unreacted NHS ester is commonly quenched or removed. The maleimide can then be coupled to thiols via thiol–maleimide addition, often requiring controlled pH and avoidance of competing thiols. Reaction efficiency depends on reagent stoichiometry, buffer composition, and thiol accessibility.

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* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C₁V₁ = C₂V₂