N-(m-PEG4)-N'-(azide-PEG4)-Cy3
CAS No.: 2107273-38-9
Cat No.: BP-501664
Purity: 98%
4.5 
N-(m-PEG4)-N'-(azide-PEG4)-Cy3 is a heterobifunctional, PEGylated cyanine dye linker designed to combine a Cy3 fluorophore with two orthogonal functional handles for PROTAC and targeted-degradation workflows. Structurally, it incorporates two short, branched polyethylene glycol segments that extend from the dye core and provide aqueous solubility and reduced nonspecific interactions, while presenting an azide group for bioorthogonal conjugation and a second PEG-linked attachment site that can be used to connect to PROTAC scaffolds or targeting ligands via compatible coupling chemistries. In PROTAC design, this type of linker is used to fluorescently tag or track degradation-related constructs, enabling visualization of cellular uptake, subcellular localization, and, when paired with appropriate partners, monitoring of ternary complex formation or degradation kinetics through fluorescence readouts. Its value lies in facilitating quantitative experimental characterization of targeted protein degradation systems while maintaining favorable solubility and minimizing perturbation of the active binding/conjugation elements.
Structure of 2107273-38-9
Assurance
Delivery
Support
* For research and manufacturing use only. Not for human or clinical use.
| Size | Price | Stock | Quantity |
|---|---|---|---|
| -- | $-- | In stock |
Looking for different specifications? Click to request a custom quote!
Capabilities & Facilities
- Comprehensive PROTAC Platform
- Scientific Expertise & Technical Support
- Custom Synthesis & Design Service
- Extensive Product Coverage
- Cutting-Edge Innovation
- Fast Delivery & Global Support
- 24/7 customer service
- 100% quality assurance
Popular Publications Citing BOC Sciences Products
This PEG-based bifunctional linker, N-(m-PEG4)-N'-(azide-PEG4)-Cy3, is designed for modular conjugation workflows commonly used in PROTAC research. By combining a Cy3 fluorescence handle with orthogonal reactive termini, it supports preparation and characterization of targeted degradation constructs while enabling tracking of conjugation efficiency and localization. The sections below describe its structure-related features and practical reactivity considerations for assembling PROTACs using PEG-mediated linkers.
Structure: The linker contains poly(ethylene glycol) segments that confer hydrophilicity and conformational flexibility, attached to the Cy3 scaffold through cyanine nitrogen substitution. An azide-functional PEG terminus provides a bioorthogonal handle, while the Cy3 moiety offers a fluorescent label for monitoring conjugation. Ether-rich PEG chains promote aqueous compatibility and reduced nonspecific interactions.
Reactivity: The azide terminus is well suited for copper-catalyzed azide–alkyne cycloaddition or strain-promoted azide–alkyne cycloaddition, enabling efficient formation of triazole-linked conjugates under commonly used bioconjugation conditions. For PROTAC assembly, select compatible alkyne-bearing warheads or ligands and perform coupling in aqueous or mixed solvent systems that maintain solubility. Copper-free approaches are often preferred when fluorescence preservation or sensitive biomolecules are involved.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
Related Product Recommendations
Please contact us with any specific requirements and we will get back to you as soon as possible.
