N-(m-PEG4)-N'-(Biotin-PEG2-amido-PEG4)-Cy5
CAS No.: 2107273-78-7
Cat No.: BP-501691
4.5 
N-(m-PEG4)-N'-(Biotin-PEG2-amido-PEG4)-Cy5 is a heterobifunctional, PEG-based linker conjugate that combines a Cy5 fluorophore with a biotin handle through defined polyethylene glycol spacers. Structurally, it features a PEG4 segment on one side and a biotin-PEG2-amido-PEG4 motif on the other, providing flexible, hydrophilic distance control between the dye and the biotin moiety while minimizing nonspecific interactions. In PROTAC and targeted protein degradation workflows, such linkers are valuable for constructing traceable conjugates and for enabling orthogonal capture or enrichment: the Cy5 label supports fluorescence-based monitoring of conjugate formation, cellular uptake, and degradation-associated trafficking, whereas the biotin moiety can be used for affinity pull-down with streptavidin-based reagents to quantify engagement or isolate complexes. Its PEG architecture typically improves solubility and reduces steric hindrance, facilitating reliable experimental readouts in mechanistic studies of targeted degradation.
Structure of 2107273-78-7
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This N-(m-PEG4)-N'-(Biotin-PEG2-amido-PEG4)-Cy5 linker is a multifunctional, PEG-based conjugation scaffold designed to support targeted protein degradation workflows. Its polyethylene glycol segments provide hydrophilicity and conformational flexibility, while the biotin handle enables strong affinity-based capture and analytical workflows. The appended Cy5 fluorophore supports tracking of PROTAC assembly and behavior in experimental systems. The following points describe its structure and reactivity in detail.
Structure: The molecule comprises multiple polyethylene glycol chains connected through amide linkages, providing a flexible, water-compatible architecture. It incorporates a biotin-derived moiety for affinity interactions and a cyanine dye core for fluorescence. The overall structure contains ether and amide functionalities that support stable conjugation chemistry.
Reactivity: This linker is suited to PROTAC construction strategies that rely on amide-forming coupling or orthogonal functional-group pairing, depending on the reactive termini presented on the partnering ligands. Typical approaches use activated carboxylic acids or amine-reactive intermediates under mild base conditions, often with coupling reagents commonly used for peptide-bond formation. Solvents such as aqueous-organic mixtures are frequently employed to maintain PEG solubility and preserve dye integrity during conjugation.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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