N-methyl-N'-methyl-O-(m-PEG4)-O'-(propargyl-PEG4)-Cy5
CAS No.: 2107273-50-5
Cat No.: BP-501520
Purity: 96%
4.5 
N-methyl-N’-methyl-O-(m-PEG4)-O’-(propargyl-PEG4)-Cy5 is a bifunctional, PEGylated Cy5 linker bearing two distinct reactive handles: an N-methylated, ether-linked PEG segment and a propargyl-PEG terminus suitable for bioorthogonal conjugation. Structurally, it consists of a cyanine dye core (Cy5) tethered through m-PEG4 spacers to provide aqueous solubility and controlled hydrophilicity, while the propargyl group enables efficient coupling to azide-bearing partners via copper-catalyzed azide–alkyne cycloaddition (CuAAC). In PROTAC and targeted degradation workflows, such linkers are valuable for generating fluorescently traceable conjugates, allowing researchers to monitor synthesis, quantify cellular uptake, and track intracellular localization of degradation constructs without disrupting the recognition elements. The PEG spacers help reduce nonspecific interactions and steric hindrance, supporting reliable assembly and characterization of dye-labeled PROTACs or related ternary-complex probes for mechanistic studies.
Structure of 2107273-50-5
Assurance
Delivery
Support
* For research and manufacturing use only. Not for human or clinical use.
| Size | Price | Stock | Quantity |
|---|---|---|---|
| -- | $-- | In stock |
Looking for different specifications? Click to request a custom quote!
Capabilities & Facilities
- Comprehensive PROTAC Platform
- Scientific Expertise & Technical Support
- Custom Synthesis & Design Service
- Extensive Product Coverage
- Cutting-Edge Innovation
- Fast Delivery & Global Support
- 24/7 customer service
- 100% quality assurance
Popular Publications Citing BOC Sciences Products
This linker can be used in PROTAC synthesis and related conjugation workflows while providing PEG-based solubility and conformational flexibility. Its functional groups support orthogonal conjugation strategies, facilitating modular assembly of degraders for mechanistic studies and optimization of cellular degradation workflows. The detailed structural and reactivity considerations are provided below to guide experimental design and linker handling.
Structure: The linker comprises a Cy5-containing scaffold bearing PEG4-based ether spacers and N-methylated cyanine nitrogen substituents, along with terminal propargyl functionality. It features ether linkages, carbon–nitrogen bonds, and an alkyne for click-type coupling, with high polarity and water-compatible behavior typical of PEG segments.
Reactivity: The propargyl terminus enables copper-catalyzed azide–alkyne cycloaddition for robust, chemoselective conjugation to azide-functional partners under standard click conditions. Assembly is typically performed in polar organic/aqueous mixtures using Cu(I) sources and stabilizing ligands to control alkyne activation. PEG-rich ether linkers generally tolerate mild conditions, supporting stepwise PROTAC construction while minimizing side reactions that can occur with less selective electrophiles.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
Related Product Recommendations
Please contact us with any specific requirements and we will get back to you as soon as possible.
