Name: 2,5-Dioxo-1-pyrrolidinyl 5-[4-(1,2,4,5-tetrazin-3-yl)benzylamino]-5-oxopentanoate
Brand: BOC Sciences
Rating: 5 (1 reviews)

Size

Price

Stock

Quantity

$--

In stock

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere.

IUPACName

(2,5-dioxopyrrolidin-1-yl) 5-oxo-5-[[4-(1,2,4,5-tetrazin-3-yl)phenyl]methylamino]pentanoate

Synonyms

InChI Key

InChI

SMILES

This tetrazine-bearing NHS ester is a dual-reactive linker for amine labeling and bioorthogonal conjugation. Its activated ester enables covalent attachment to amine-containing ligands, while the tetrazine unit supports rapid IEDDA ligation with strained alkene partners. The molecule is described in detail below.

Structure: The molecule contains a 1,2,4,5-tetrazine-substituted benzyl amide connected to a glutarate-derived N-hydroxysuccinimide ester. The aromatic tetrazine segment provides bioorthogonal reactivity, while the succinimidyl ester serves as an amine-reactive acylating group for linker installation.

Reactivity: The NHS ester reacts with primary amines under mildly basic, anhydrous or buffered conditions to form stable amide bonds. The tetrazine moiety then undergoes inverse-electron-demand Diels-Alder ligation with strained alkene partners such as TCO. Avoid nucleophilic buffers during ester coupling, and protect the tetrazine from strong reductants or prolonged harsh conditions.

Stock concentration: *

Desired final volume: *

Desired concentration: *

* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C₁V₁ = C₂V₂