Biotin-PEG23-azide
Cat No.: BP-501818
4.5 
Biotin-PEG23-azide is a heterobifunctional PEG-based linker that combines a biotin affinity handle with a terminal azide for efficient, chemoselective conjugation. Structurally, it consists of a long polyethylene glycol chain that provides aqueous solubility and conformational flexibility, linking a biotin moiety to an azide group through a stable ether/PEG linkage. In PROTAC and targeted degradation workflows, the azide functionality enables modular attachment to alkyne-bearing partners via copper-free or copper-catalyzed azide–alkyne cycloaddition, allowing researchers to incorporate this linker into degraders, probes, or control constructs while preserving the activity of the binding and E3-recruiting elements. The biotin end can be used for affinity enrichment, immobilization on streptavidin matrices, or detection of conjugates, facilitating characterization of degradation reagents and optimization of linker placement. Its PEG spacer length and chemical orthogonality make it a practical tool for assembling and validating targeted protein degradation systems with improved handling and experimental throughput.
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* For research and manufacturing use only. Not for human or clinical use.
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Biotin-PEG23-azide is a PEG-based bifunctional linker designed for modular assembly of PROTACs and related targeted degradation constructs. Its biotin handle enables strong, affinity-based capture and convenient conjugation workflows, while the azide group supports robust, bioorthogonal coupling to compatible partners. The flexible polyethylene glycol spacer is expected to enhance solubility and reduce steric constraints, supporting efficient formation of ternary complexes in targeted protein degradation studies. The detailed structural and reactivity features are described below.
Structure: Biotin-PEG-azide comprises a biotin moiety tethered to a long, flexible polyethylene glycol chain terminating in an azide functional group. The linker contains ether linkages within the PEG segment and an azide substituent suitable for click-type reactions. Overall polarity and conformational flexibility promote aqueous compatibility.
Reactivity: The azide functionality is well suited for copper-catalyzed azide–alkyne cycloaddition or strain-promoted azide–alkyne cycloaddition, depending on the alkyne partner. For copper-catalyzed routes, typical conditions use a Cu(I) source generated in situ with appropriate ligands, in aqueous or mixed solvents, to drive efficient triazole formation. Mechanistically, the reaction proceeds via azide activation and cycloaddition to form a stable conjugation linkage under mild, PROTAC-compatible handling.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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