1. Effects of bioleaching pretreatment on nitrous oxide emission related functional genes in sludge composting process
Yiqi Wu, Ke Wang, Chao He, Zhe Wang, Nanqi Ren, Yu Tian Bioresour Technol. 2018 Oct;266:181-188.doi: 10.1016/j.biortech.2018.06.049.Epub 2018 Jun 19.
The effect of bioleaching pretreatment on N2O generation in sludge composting process was firstly investigated in this study. The relationships among physicochemical factors, N2O and NH3 emission and related functional genes were analyzed in 60 days composting of bioleaching dewatering sludge (BDS) and filter press dewatering sludge (FDS), respectively. The results showed the cumulative amounts of NH3 and N2O emission from the BDS composting system were reduced by 83.52% and 54.76% after bioleaching pretreatment, respectively. The lower moisture and pH, and the higher ORP and the concentrations of NH4+-N, NO3--N and NO2--N were observed in BDS during the composting compared to FDS. Furthermore, bioleaching pretreatment improved the relative abundance of hao but reduced amoA, nirK and norB in the BDS during the composting. The low pH level and the reduction of nirK and norB in BDS were the main reasons mitigating NH3 and N2O emissions, respectively.
2. NF-κB Signaling-Mediated Activation of WNK-SPAK-NKCC1 Cascade in Worsened Stroke Outcomes of Ang II-Hypertensive Mice
Mohammad Iqbal H Bhuiyan, Cullen B Young, Israt Jahan, Md Nabiul Hasan, Sydney Fischer, Nur Farah Meor Azlan, Mingjun Liu, Ansuman Chattopadhyay, Huachen Huang, Kristopher T Kahle, Jinwei Zhang, Samuel M Poloyac, Bradley J Molyneaux, Adam C Straub0, Xianming Deng, Delphine Gomez0, Dandan Sun Stroke. 2022 May;53(5):1720-1734.doi: 10.1161/STROKEAHA.121.038351.Epub 2022 Mar 11.
Background:Worsened stroke outcomes with hypertension comorbidity are insensitive to blood pressure-lowering therapies. In an experimental stroke model with comorbid hypertension, we investigated causal roles of ang II (angiotensin II)-mediated stimulation of the brain WNK (with no lysine [K] kinases)-SPAK (STE20/SPS1-related proline/alanine-rich kinase)-NKCC1 (Na-K-Cl cotransporter) complex in worsened outcomes.
3. Genetic Testing in Natural History Studies: A Review of the Regulatory and Legal Landscape
Andrew Bevan, Delphine Saragoussi, Laura Sayegh, Moira Ringo, Fiona Kearney Public Health Genomics. 2021;24(3-4):75-88.doi: 10.1159/000514208.Epub 2021 Mar 23.
Background:Natural history (NH) studies, using observational methods, are common in rare and orphan diseases (80% of which have a genetic component). There is profound interest in identifying genetic mutations driving these diseases in these studies to support the formulation of targeted precision medicines. The global regulatory classification of NH studies with novel molecular biomarker collection has not been clearly delineated, presenting researchers with the challenge of determining how these studies are classified and regulated across multiple geographies. Objective:The aim of this investigation was to conduct a review of regulations related to NH studies and genetic testing to elucidate regulatory pathways to inform clinical researchers in the field.
