Pomalidomide-C3-NH2 hydrochloride

Background Introduction

Pomalidomide-C3-NH2 hydrochloride is a specialized E3 ligase ligand-linker conjugate. As a derivative of pomalidomide, it is engineered for use in targeted protein degradation strategies, particularly for the design and synthesis of PROTACs (Proteolysis Targeting Chimeras). This compound provides a critical building block for research focused on harnessing the ubiquitin-proteasome system for selective protein degradation.

Mechanism

Pomalidomide-C3-NH2 hydrochloride functions by binding to the E3 ubiquitin ligase cereblon (CRBN), thanks to its pomalidomide moiety. The attached C3 linker, terminated with an amine group (NH2), facilitates conjugation with various target protein ligands through amide bond formation or other linkage strategies. In a PROTAC construct, this enables the recruitment of CRBN to the target protein, resulting in its ubiquitination and subsequent proteasomal degradation, effectively removing disease-relevant or pathological proteins from cells.

Applications

Pomalidomide-C3-NH2 hydrochloride is widely used in PROTAC development workflows to create novel therapeutics and research tools. Its applications include constructing bifunctional molecules for targeted protein degradation, enabling the study of protein function, and the validation of new drug targets in oncology, neurodegeneration, immunology, and other therapeutic areas. This conjugate is also suitable for high-throughput screening, medicinal chemistry optimization, and the generation of chemical probes for biological research.