Tetrazine-Ph-PEG5-NHS ester
CAS No.: 1682653-80-0
Cat No.: BP-501097
Purity: 95%
4.5 
Tetrazine-Ph-PEG5-NHS ester is a bifunctional PEG-based linker designed for PROTAC and related targeted degradation workflows that require robust, site-selective conjugation. Structurally, it combines a tetrazine moiety for rapid inverse-electron-demand Diels–Alder reactivity with a phenyl spacer and a five-unit poly(ethylene glycol) chain terminating in an N-hydroxysuccinimide (NHS) ester. The NHS ester enables efficient coupling to primary amines on lysine residues or engineered tags of targeting ligands (e.g., small-molecule binders or peptides), while the tetrazine group can subsequently react with complementary trans-cyclooctene or strained-alkene partners to form a stable conjugation under mild conditions. This architecture helps tune linker length and hydrophilicity, improving solubility and reducing non-specific interactions, while providing modular assembly of multi-component degraders. The reagent is therefore valuable for constructing well-defined PROTACs and for optimizing conjugation strategies in targeted protein degradation research.
Structure of 1682653-80-0
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Tetrazine-Ph-PEG5-NHS ester is a PEG-based linker engineered for modular PROTAC construction, enabling efficient conjugation of targeting ligands to tetrazine-bearing handles for bioorthogonal assembly. Its flexible polyethylene glycol scaffold improves solubility and conformational freedom, while the NHS ester supports rapid amide formation under standard bioconjugation conditions. This product is well suited for researchers designing targeted protein degradation workflows, where subsequent steps require reliable, controllable linkage chemistry; detailed structural and reactivity considerations are provided below.
Structure: The linker combines a tetrazine functional group with a phenyl spacer and a polyethylene glycol chain terminating in an N-hydroxysuccinimide ester. It contains aromatic rings, ether linkages within the PEG backbone, and an activated carboxyl-derived amide-forming moiety, supporting aqueous compatibility and stable conjugation chemistry.
Reactivity: The NHS ester reacts with primary amines to form amide bonds via nucleophilic acyl substitution, typically using mildly basic conditions to promote amine deprotonation while preserving tetrazine integrity. Common solvents include aqueous buffers compatible with NHS chemistry, often with controlled organic co-solvents when needed. After amide coupling, tetrazine-containing intermediates can participate in bioorthogonal ligation with suitable partners under conditions that maintain tetrazine reactivity and minimize side reactions.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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