Biotin-PEG12-Mal
Cat No.: BP-502120
4.5 
Biotin-PEG12-Mal is a heterobifunctional PROTAC linker featuring a biotin moiety connected through a long, hydrophilic polyethylene glycol chain to a terminal maleimide group. The PEG12 spacer provides conformational flexibility and improves aqueous solubility, while the maleimide enables selective conjugation to thiol-containing partners via Michael-type addition, forming a stable thioether linkage. In targeted protein degradation workflows, this linker can be used to incorporate a biotin handle for affinity capture or enrichment of PROTAC assemblies, while the maleimide serves as a reactive “attachment point” to couple the linker to cysteine-bearing ligands, scaffolds, or protein-derived components. Its modular design supports experimental optimization of multicomponent degraders, facilitates pull-down and downstream analysis, and helps researchers track or isolate specific conjugates during synthesis and characterization.
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* For research and manufacturing use only. Not for human or clinical use.
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Biotin-PEG12-Mal is a biotin-functionalized polyethylene glycol linker bearing a maleimide handle, designed for modular PROTAC construction and related targeted conjugation workflows. The PEG segment provides conformational flexibility and improved solubility, while the maleimide enables efficient chemoselective coupling to thiol-containing ligands under mild conditions. The biotin moiety further supports affinity-based capture and characterization of assembled conjugates. Detailed structural and reactivity considerations are provided below.
Structure: The linker comprises a biotin group connected through a PEG chain to a maleimide electrophile. It contains a cyclic imide (maleimide) for thiol reactivity, ether linkages within the PEG for hydrophilicity, and stable carbon–carbon and carbon–heteroatom bonds that support robust conjugate formation.
Reactivity: Maleimide groups typically undergo rapid Michael-type addition with free thiols to form stable thioether linkages. For PROTAC assembly, thiol-bearing components are reacted with the maleimide under mildly basic, aqueous-compatible conditions to maintain thiol nucleophilicity while minimizing side reactions such as hydrolysis. Commonly used solvents include buffered aqueous media; catalysts are generally unnecessary, and careful control of pH and thiol stoichiometry is recommended for reproducible coupling.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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