Biotin-PEG36-PFP ester
Cat No.: BP-501929
4.5 
Biotin-PEG36-PFP ester is a heterobifunctional, polyethylene glycol–based linker that combines a biotin affinity handle with a terminal pentafluorophenyl (PFP) ester for amide-bond formation. Structurally, it consists of a long PEG chain that provides water solubility and conformational flexibility, separating the biotin moiety from the reactive ester to reduce steric interference. In PROTAC and targeted degradation workflows, this linker is useful for conjugating a ligand or protein-reactive component bearing an amine to a biotin-tagged partner, enabling controlled assembly of multi-component degraders or recruitment platforms. The PFP ester reacts efficiently with primary amines under standard coupling conditions to form stable amide linkages, while the biotin group supports strong, noncovalent capture by streptavidin or avidin for purification, immobilization, or assay readouts. Its long PEG spacer and orthogonal functional groups make it valuable for constructing modular targeted degradation reagents and for optimizing conjugation efficiency and experimental workflow.
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* For research and manufacturing use only. Not for human or clinical use.
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Biotin-PEG36-PFP ester is a polyethylene glycol-based PROTAC linker designed to connect a biotin-tagged recognition module to a reactive pentafluorophenyl (PFP) ester handle. Its PEG segment provides conformational flexibility and improved solubility, while the PFP ester enables efficient, chemoselective coupling to nucleophilic groups commonly used in targeted protein degradation workflows. The detailed structural and reactivity considerations are provided below.
Structure: The linker comprises a biotin moiety tethered through a long PEG chain to a pentafluorophenyl ester. It contains ether linkages within the PEG backbone and an activated aromatic ester, offering a stable scaffold for conjugation and subsequent PROTAC assembly under standard organic synthesis conditions.
Reactivity: The PFP ester reacts with primary amines, hydrazines, or other nucleophiles via acyl substitution, generating a stable amide or related linkage while releasing pentafluorophenol. Coupling is typically performed under mild, anhydrous-to-semi-anhydrous conditions using polar aprotic solvents and bases that promote nucleophile deprotonation. Reaction progress is commonly monitored by analytical chromatography, and the resulting conjugate is purified to remove small-molecule byproducts.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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