Biotin-PEG6-amine
Cat No.: BP-501853
Purity: >97%
4.5 
Biotin-PEG6-amine is a heterobifunctional PEG-based linker featuring a biotin moiety at one terminus and a primary amine at the other, connected through a six-unit polyethylene glycol chain that provides water solubility and conformational flexibility. In PROTAC and targeted protein degradation workflows, this linker is commonly used to install a biotin handle for affinity capture or detection (e.g., streptavidin-based enrichment and pull-down), while the terminal amine enables straightforward conjugation to activated carboxyl groups, isothiocyanates, or other electrophiles used to couple to ligands and warheads. The PEG spacer helps reduce steric interference between the biotin tag and the functional conjugate, improving accessibility during binding and analytical steps. As a versatile attachment reagent, it supports robust characterization of ternary-complex formation, facilitates workflow-compatible purification of PROTAC constructs, and enables modular assembly of biotin-tagged degradation tools for mechanistic studies.
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* For research and manufacturing use only. Not for human or clinical use.
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Biotin-PEG6-amine is a PEG-based linker bearing a terminal primary amine and a biotin handle, designed to enable modular conjugation in targeted protein degradation workflows. Its flexible, hydrophilic polyethylene glycol segment supports favorable solubility and linker mobility, while the biotin moiety provides a versatile affinity tag for biotin-mediated capture or assay integration. The terminal amine enables straightforward coupling to activated carboxylates or other electrophiles to assemble PROTAC constructs. Detailed structural and reactivity considerations follow below.
Structure: The linker contains a biotin scaffold connected to a polyethylene glycol chain terminating in a primary amine. It features ether linkages within the PEG segment, heteroatoms that enhance polarity and hydrogen-bonding, and an amine functional group for nucleophilic reactivity. Overall, it is expected to be water-compatible and conformationally flexible.
Reactivity: The terminal primary amine is suitable for amide-bond formation via coupling to activated carboxylic acids (for example, using common peptide-coupling chemistries) under standard organic synthesis conditions. Alternatively, it can participate in reductive amination or nucleophilic substitution when appropriate electrophiles are used. Typical approaches employ polar aprotic solvents with base to promote amine activation and minimize side reactions, consistent with established PROTAC linker-conjugation strategies.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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