Biotin-PEG4-hydrazide
CAS No.: 756525-97-0
Cat No.: BP-501433
Purity: >95%
4.5 
Biotin-PEG4-hydrazide is a heterobifunctional PEG-based linker that combines a biotin handle with a hydrazide reactive group through a short, flexible polyethylene glycol spacer. The PEG4 segment provides aqueous solubility and spatial separation, reducing steric interference when the linker is incorporated into larger conjugates. In PROTAC and targeted degradation workflows, the biotin moiety can be used to enable affinity capture or modular attachment to biotin-binding reagents, while the hydrazide group serves as a carbonyl-reactive handle for forming hydrazone linkages with appropriately functionalized partners. This design supports the assembly of degraders or intermediate conjugates by allowing controlled, reversible coupling strategies that can preserve the binding properties of targeting ligands and E3-recruiting modules. As a practical tool for researchers, it facilitates purification, immobilization, and stepwise construction of multicomponent degradation systems, improving experimental flexibility and enabling systematic optimization of linker length and conjugation chemistry.
Structure of 756525-97-0
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Biotin-PEG4-hydrazide is a polyethylene glycol–based linker designed to connect biotin-tagged recognition elements to hydrazide-reactive partners in PROTAC and related targeted degradation workflows. Its PEG-rich scaffold provides aqueous compatibility and conformational flexibility, helping maintain productive ternary complex formation. The hydrazide functionality enables controlled coupling strategies for assembling degraders, and the biotin handle supports orthogonal affinity-based detection or enrichment. Detailed structural and reactivity considerations are provided below.
Structure: The linker contains a PEG chain that confers hydrophilicity and segmental flexibility, terminated by a hydrazide group. Amide and hydrazide functional groups provide hydrogen-bonding capacity, while the ether-rich PEG backbone supports solvation and reduced nonspecific aggregation in aqueous media.
Reactivity: The hydrazide moiety is suitable for condensation-based coupling to carbonyl-containing partners, including aldehydes or activated derivatives, forming hydrazone linkages under mildly acidic conditions. In PROTAC assembly, this enables modular attachment while preserving aqueous compatibility. Typical approaches use polar organic solvents or buffered aqueous systems, with reaction progress monitored by chromatographic or spectrometric methods; catalysts are chosen to promote condensation without degrading sensitive warheads.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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