Name: DBCO-Biotin
Brand: BOC Sciences
Rating: 5 (1 reviews)

Size

Price

Stock

Quantity

$--

In stock

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere.

IUPACName

5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[3-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-3-oxopropyl]pentanamide

Synonyms

Azadibenzocyclooctyne-Biotin conjugate; N-[3-(11,12-Didehydrodibenzo[b,f]azocin-5(6H)-yl)-3-oxopropyl]-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide

Boiling Point

905.9±65.0 °C at 760 mmHg

Density

1.4±0.1 g/cm3

InChI Key

ODZDNKWGVJPVBP-DPPGTGKWSA-N

InChI

InChI=1S/C28H30N4O3S/c33-25(12-6-5-11-24-27-22(18-36-24)30-28(35)31-27)29-16-15-26(34)32-17-21-9-2-1-7-19(21)13-14-20-8-3-4-10-23(20)32/h1-4,7-10,22,24,27H,5-6,11-12,15-18H2,(H,29,33)(H2,30,31,35)/t22-,24-,27-/m0/s1

SMILES

C1C2C(C(S1)CCCCC(=O)NCCC(=O)N3CC4=CC=CC=C4C#CC5=CC=CC=C53)NC(=O)N2

1. Liposome Click Membrane Permeability Assay for Identifying Permeable Peptides

Tanvi J Desai, Bahanu Habulihaz, Joe R Cannon, Arun Chandramohan, Hung Yi Kristal Kaan, Ahmad Sadruddin, Tsz Ying Yuen, Charles Johannes, Dawn Thean, Chris J Brown, David P Lane, Anthony W Partridge, Raymond Evers, Tomi K Sawyer, Jerome Hochman Pharm Res. 2021 May;38(5):843-850. doi: 10.1007/s11095-021-03005-z.Epub 2021 Mar 15.

Purpose:To develop a novel, target agnostic liposome click membrane permeability assay (LCMPA) using liposome encapsulating copper free click reagent dibenzo cyclooctyne biotin (DBCO-Biotin) to conjugate azido modified peptides that may effectively translocate from extravesicular space into the liposome lumen.Method:DBCO-Biotin liposomes were prepared with egg phosphatidylcholine and cholesterol by lipid film rehydration, freeze/thaw followed by extrusion. Size of DBCO-Biotin liposomes were characterized with dynamic light scattering. Results:The permeable peptides representing energy independent mechanism of permeability showed higher biotinylation in LCMPA. Individual peptide permeability results from LCMPA correlated well with shifts in potency in cellular versus biochemical assays (i.e., cellular/ biochemical ratio) demonstrating quantitative correlation to intracellular barrier in intact cells.Conclusion:The study provides a novel membrane permeability assay that has potential to evaluate energy independent transport of diverse peptides.

Can DBCO-Biotin be discussed as a research-grade intermediate?

Yes. It can be discussed as a research-grade intermediate for fine chemical, and research work, especially where the project needs a defined structure for synthesis screening or sourcing evaluation. The final quotation should be based on current availability, quality requirements, and requested quantity.

14/09/2025

Is COA or SDS available for this product?

COA, SDS, or other analytical documentation may be available depending on batch availability and project requirements. Please contact us to confirm the available documents.

06/04/2025

Can this product be supplied in different quantities?

Package size and supply scale can be discussed based on current availability, production schedule, and project requirements. Please submit your required quantity for evaluation.

27/12/2023

Can I request a quote for DBCO-Biotin?

Yes. Please submit the product name, CAS number, required quantity, desired purity, destination country, and expected timeline so that availability and quotation details can be reviewed.

16/11/2023

What documentation should be discussed for DBCO-Biotin orders?

Depending on batch availability and project requirements, documentation may include COA, SDS, chromatographic data, or other analytical information. Please specify which documents are needed for internal review, import clearance, or project qualification when submitting the inquiry.

12/04/2022

Stock concentration: *

Desired final volume: *

Desired concentration: *

* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C₁V₁ = C₂V₂