Name: DSPE-PEG-Biotin sodium salt
Brand: BOC Sciences
Rating: 5 (1 reviews)

Size

Price

Stock

Quantity

$--

In stock

Purity

≥95%

Solubility

Soluble in chloroform, warm water (for powder form).

Appearance

Off-white/white solid or liquid.

ShelfLife

1 Year.

Storage

Store at -20 °C

Shipping

Shipped under RT.

Synonyms

Poly(oxy-1,2-ethanediyl), α-[(9R)-6-hydroxy-6-oxido-1,12-dioxo-9-[(1-oxooctadecyl)oxy]-5,7,11-trioxa-2-aza-6-phosphanonacos-1-yl]-ω-[2-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]ethoxy]-, sodium salt (1:1); Poly(oxy-1,2-ethanediyl), α-[(9R)-6-hydroxy-6-oxido-1,12-dioxo-9-[(1-oxooctadecyl)oxy]-5,7,11-trioxa-2-aza-6-phosphanonacos-1-yl]-ω-[2-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]ethoxy]-, monosodium salt; α-[(9R)-6-Hydroxy-6-oxido-1,12-dioxo-9-[(1-oxooctadecyl)oxy]-5,7,11-trioxa-2-aza-6-phosphanonacos-1-yl]-ω-[2-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]ethoxy]poly(oxy-1,2-ethanediyl) sodium salt (1:1); 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000]; DSPE-PEG-Biotin sodium salt; Laysan Bio DSPE-PEG-biotin

1. Corrections to "Targeted Microbubble Mediated Sonoporation of Endothelial Cells In Vivo"

Ilya Skachkov, Ying Luan, Antonius F W van der Steen, Nico de Jong, Klazina Kooiman IEEE Trans Ultrason Ferroelectr Freq Control. 2021 Jun;68(6):2320.doi: 10.1109/TUFFC.2021.3064763.Epub 2021 May 25.

In the above article [1], the authors regret that there was a mistake in calculating the mol% of the microbubble coating composition. For all experiments, the unit in mg/mL was utilized and the conversion mistake only came when converting to mol% in order to define the ratio between the coating formulation components. The correct molecular weight of PEG-40 stearate is 2046.54 g/mol [2], [3], not 328.53 g/mol. On page 1661, paragraph II-A, it should read "The coating was composed of DSPC (84.8 mol%; P 6517; Sigma-Aldrich, Zwijndrecht, The Netherlands);PEG-40 stearate (8.2 mol%; P 3440; Sigma-Aldrich); DSPE-PEG(2000) (5.9 mol%; 880125 P; Avanti Polar Lipids, Alabaster, AL, USA); and DSPE-PEG(2000)-biotin (1.1 mol%; 880129 C; Avanti Polar Lipids)".

2. Biotinylated lipid bilayer disks as model membranes for biosensor analyses

Anna Lundquist, Søren B Hansen, Helena Nordström, U Helena Danielson, Katarina Edwards Anal Biochem. 2010 Oct 15;405(2):153-9.doi: 10.1016/j.ab.2010.06.030.Epub 2010 Jun 19.

The aim of this study was to investigate the potential of polyethylene glycol (PEG)-stabilized lipid bilayer disks as model membranes for surface plasmon resonance (SPR)-based biosensor analyses. Nanosized bilayer disks that included 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)(2000)] (DSPE-PEG(2000)-biotin) were prepared and structurally characterized by cryo-transmission electron microscopy (cryo-TEM) imaging. The biotinylated disks were immobilized via streptavidin to three different types of sensor chips (CM3, CM4, and CM5) varying in their degree of carboxymethylation and thickness of the dextran matrix. The bilayer disks were found to interact with and bind stably to the streptavidin-coated sensor surfaces. As a first step toward the use of these bilayer disks as model membranes in SPR-based studies of membrane proteins, initial investigations were carried out with cyclooxygenases 1 and 2 (COX 1 and COX 2). Bilayer disks were preincubated with the respective protein and thereafter allowed to interact with the sensor surface. The signal resulting from the interaction was, in both cases, significantly enhanced as compared with the signal obtained when disks alone were injected over the surface. The results of the study suggest that bilayer disks constitute a new and promising type of model membranes for SPR-based biosensor studies.

3. Corrections to "Vibrational Responses of Bound and Nonbound Targeted Lipid-Coated Single Microbubbles"

Tom van Rooij, Ines Beekers, Kirby R Lattwein, Antonius F W van der Steen, Nico de Jong, Klazina Kooiman IEEE Trans Ultrason Ferroelectr Freq Control. 2021 Jun;68(6):2319.doi: 10.1109/TUFFC.2021.3064751.Epub 2021 May 25.

In the above article [1], the authors regret that there was a mistake in calculating the mol% of the microbubble coating composition. For all experiments, the unit in mg/mL was utilized and the conversion mistake only came when converting to mol% in order to define the ratio between the coating formulation components. The correct molecular weight of PEG-40 stearate is 2046.54 g/mol [2], [3], not 328.53 g/mol. On page 786, paragraph II-A, it should read "The coating was composed of 84.8 mol% DSPC (P6517, Sigma-Aldrich, Zwijndrecht, The Netherlands) or DPPC (850355, Avanti Polar Lipids, Alabaster, AL, USA); 8.2 mol% polyoxyethylene-40-stearate (PEG-40 stearate, P3440, Sigma-Aldrich); 5.9 mol% 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] (DSPE-PEG(2000), 880125, Avanti Polar Lipids); and 1.1 mol% 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000] (DSPE-PEG(2000)-biotin, 880129, Avanti Polar Lipids)."

DSPE-PEG (2000) Biotin is a lipid–polyethylene glycol conjugate designed to serve as a functional linker in targeted protein degradation workflows, enabling biotin-mediated handling and modular assembly of PROTAC-related components. Its amphiphilic DSPE anchor supports membrane or hydrophobic association, while the PEG spacer provides aqueous compatibility and reduced non-specific interactions. This product’s biotin functionality facilitates affinity-based capture and controlled positioning of targeting modules; detailed structural and reactivity considerations are provided below.

Structure: The molecule combines a phospholipid (DSPE) hydrophobic tail with a PEG ether chain and terminal biotin. It contains ester and ether linkages, a phosphodiester headgroup, and multiple heteroatoms that promote hydrogen bonding and water solubility. Overall, it is an amphiphilic conjugate with flexible PEG-mediated spacing.

Reactivity: For PROTAC construction, this linker is typically used in affinity-enabled assembly strategies rather than as a high-reactivity small-molecule coupling partner. Biotin can be exploited for strong streptavidin/avidin binding to organize components, while DSPE supports incorporation into lipid-containing systems. Assembly is commonly performed in buffered aqueous media under gentle mixing; covalent coupling, when required, is generally performed by reacting complementary functional groups on partner molecules using standard amide or thiol–maleimide chemistries with appropriate catalysts and bases.

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Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C₁V₁ = C₂V₂