Name: Hydroxy-PEG3-t-butyl ester
Brand: BOC Sciences
Rating: 5 (1 reviews)

Size

Price

Stock

Quantity

$--

In stock

Purity

≥95%

Solubility

Soluble in DMSO (10 mm)

Appearance

Colorless to light yellow to light orange clear liquid

Storage

Store at 2-8°C

Shipping

Room temperature, or blue ice upon request.

IUPACName

tert-butyl 3-[2-[2-(2-hydroxyethoxy)ethoxy]ethoxy]propanoate

Synonyms

HO-PEG3-CH2CH2COOtBu; Hydroxy-PEG3-(CH2)2-Boc; OH-PEG3-TBA; OH-PEG3-CH2CH2COOtBu; HO-PEG3-t-butyl ester; tert-Butyl 12-hydroxy-4,7,10-trioxadodecanoate; 3-[2-[2-(2-Hydroxyethoxy)ethoxy]ethoxy]propionic acid tert-butyl ester; 12-Hydroxy-4,7,10-trioxadodecanoic Acid tert-Butyl Ester; 2-Methyl-2-propanyl 3-{2-[2-(2-hydroxyethoxy)ethoxy]ethoxy}propanoate; Propanoic acid, 3-[2-[2-(2-hydroxyethoxy)ethoxy]ethoxy]-, 1,1-dimethylethyl ester; tert-Butyl triethylene glycol-O-propionate

Boiling Point

365.8±27.0°C (Predicted)

Density

1.052 g/mL at 20°C

InChI Key

KSXVEOLRERRELV-UHFFFAOYSA-N

InChI

InChI=1S/C13H26O6/c1-13(2,3)19-12(15)4-6-16-8-10-18-11-9-17-7-5-14/h14H,4-11H2,1-3H3

SMILES

CC(C)(C)OC(=O)CCOCCOCCOCCO

Biological Activity

Hydroxy-PEG2-(CH2)2-Boc is a uncleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). Hydroxy-PEG2- (CH2)2-Boc is extracted from patent WO2004008101A2 (compound 196)[1] . In Vitro: ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.

Hydroxy-PEG3-t-butyl ester is a polyethylene glycol (PEG)-based PROTAC linker building block designed to provide aqueous compatibility, conformational flexibility, and tunable spacing between ligands. Its PEG segment supports favorable solubility and reduced nonspecific interactions, while the protected carboxylate functionality enables controlled downstream conjugation to assemble targeted protein degraders. The structure and reactivity considerations for constructing PROTACs are described in detail below.

Structure: The linker contains an ether-rich PEG chain featuring a terminal hydroxyl group and a tert-butyl ester-protected carboxylate. It includes polar ether linkages, an ester carbonyl, and a tert-butyl group that masks the acidic functionality. Overall, it exhibits high hydrophilicity and flexible conformational behavior.

Reactivity: The tert-butyl ester is suitable for acid-mediated deprotection to reveal a reactive carboxylic acid for amide or ester coupling to PROTAC ligands. Typical construction strategies involve activating the liberated acid using standard coupling chemistries and forming stable linkages under mild, anhydrous conditions. Deprotection commonly uses dilute acid in organic or mixed solvents, followed by base-neutralization and purification prior to conjugation.

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* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C₁V₁ = C₂V₂