Mal-NO2-Ph-PEG12-NHS
Cat No.: BP-501990
4.5 
Mal-NO2-Ph-PEG12-NHS is a heterobifunctional PEG-based linker designed for PROTAC and targeted protein degradation workflows, combining a maleimide handle with an NHS ester for conjugation to complementary functional groups. The structure incorporates a nitro-substituted phenyl spacer that can tune linker hydrophobicity and spatial presentation, while the extended PEG chain provides conformational flexibility and improves aqueous solubility, helping maintain productive geometry between the two PROTAC modules. In experimental PROTAC assembly, the maleimide group selectively reacts with thiols (e.g., cysteine-containing ligands or engineered protein-binding modules), whereas the NHS ester forms stable amide bonds with primary amines on the partner component. This dual-reactivity enables controlled, stepwise coupling to generate well-defined conjugates for recruiting an E3 ligase and the target-binding moiety. The resulting linker is valuable for researchers seeking modular, reproducible synthesis of degraders with adjustable spacing and improved handling during optimization studies.
Assurance
Delivery
Support
* For research and manufacturing use only. Not for human or clinical use.
| Size | Price | Stock | Quantity |
|---|---|---|---|
| -- | $-- | In stock |
Looking for different specifications? Click to request a custom quote!
Capabilities & Facilities
- Comprehensive PROTAC Platform
- Scientific Expertise & Technical Support
- Custom Synthesis & Design Service
- Extensive Product Coverage
- Cutting-Edge Innovation
- Fast Delivery & Global Support
- 24/7 customer service
- 100% quality assurance
Popular Publications Citing BOC Sciences Products
This PROTAC linker, Mal-NO2-Ph-PEG12-NHS, is designed to connect a maleimide-bearing module to an amine-reactive NHS ester, enabling modular assembly of targeted protein degraders. Its PEG-based spacer provides conformational flexibility and improved linker solubility, while the maleimide functionality supports selective conjugation to thiol-containing ligands. The aromatic nitro-substituted phenyl segment offers a defined hydrophobic handle for stable linker presentation. The structure and reactivity parameters are described in detail below.
Structure: The linker combines a PEG chain with a maleimide group and an NHS ester, incorporating an aromatic nitro-substituted phenyl segment. It contains carbon–carbon and carbon–heteroatom linkages, with electrophilic centers on the maleimide and NHS ester. The PEG segment confers hydrophilicity and flexible spacing.
Reactivity: The NHS ester reacts with primary amines to form stable amide bonds under mildly basic aqueous conditions, typically using buffering systems that maintain NHS reactivity. The maleimide undergoes thiol–maleimide Michael addition with cysteine or other thiol-bearing partners, often in near-neutral buffers to preserve maleimide selectivity. Common solvents include water or aqueous organic mixtures; purification is usually performed by chromatography or dialysis to remove unreacted components.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
Related Product Recommendations
Please contact us with any specific requirements and we will get back to you as soon as possible.
