Biotin-PEG2-NHS ester
CAS No.: 596820-83-6
Cat No.: BP-500268
Purity: >97%
4.5 
Biotin-PEG2-NHS ester is a heterobifunctional PEG-based linker that combines a biotin affinity handle with an N-hydroxysuccinimide (NHS) ester for amide-bond formation. Structurally, it contains a short polyethylene glycol spacer that provides aqueous solubility and reduces steric hindrance between the biotin moiety and the biomolecule to be coupled. In PROTAC and targeted protein degradation workflows, this reagent is used to functionalize proteins, peptides, or other targeting components bearing primary amines, enabling subsequent conjugation to biotin-binding partners such as streptavidin or avidin-coated surfaces and reagents. The PEG spacer helps maintain accessibility of the biotin for affinity capture while the NHS ester facilitates efficient, site-selective attachment to lysine residues or N-termini. Its value lies in supporting modular assembly, purification, and detection of degradation constructs, including experiments that require robust immobilization or enrichment of PROTAC-related intermediates.
Structure of 596820-83-6
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Biotin-PEG2-NHS ester is a PEG-based, NHS-activated linker designed to enable efficient conjugation of biotinylated constructs for targeted protein degradation workflows. Its PEG spacer provides aqueous compatibility and flexible presentation of the biotin handle, while the NHS ester supports robust amide-bond formation with primary amines. This makes it useful for assembling PROTAC-related reagents where biotin tagging, affinity capture, or modular linker installation is required. Detailed structural and reactivity considerations are provided below.
Structure: The molecule comprises a biotin moiety connected through a short polyethylene glycol spacer to an N-hydroxysuccinimide ester. It contains an activated carboxylate (NHS ester) for acyl transfer, along with ether linkages in the PEG segment and amide-forming functionality upon reaction.
Reactivity: The NHS ester reacts with primary amines to form stable amide bonds via nucleophilic acyl substitution, typically under mildly basic conditions that preserve NHS reactivity. Common approaches use aqueous or mixed aqueous buffers with controlled pH, with the amine-containing partner added to drive coupling. No special catalysts are generally required; careful buffer selection and avoidance of competing nucleophiles help maintain coupling efficiency.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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