DBCO-NHCO-PEG12-biotin
Cat No.: BP-502112
4.5 
DBCO-NHCO-PEG12-biotin is a heterobifunctional, PEG-based linker that combines a dibenzocyclooctyne (DBCO) cyclooctyne handle with a biotin affinity tag through an amide-linked polyethylene glycol spacer. Structurally, it features a DBCO moiety for strain-promoted azide–alkyne cycloaddition (SPAAC) and a PEG chain of extended length that provides aqueous solubility and spatial flexibility, reducing steric interference between conjugated partners. In PROTAC and targeted protein degradation workflows, the DBCO group enables efficient, catalyst-free coupling to azide-functionalized ligands or intermediate constructs, allowing modular assembly of degraders from separately prepared components. The terminal biotin facilitates downstream capture, enrichment, and analytical tracking using streptavidin or avidin-based systems, supporting optimization of conjugation efficiency and ternary complex–related experiments. This reagent is valuable for researchers seeking robust, bio-orthogonal linker chemistry and convenient affinity handles to streamline degrader synthesis and characterization in vitro.
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* For research and manufacturing use only. Not for human or clinical use.
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- Comprehensive PROTAC Platform
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This DBCO-NHCO-PEG12-biotin linker is designed to support efficient, modular assembly of PROTACs by combining a strain-promoted alkyne handle with a biotin tag and a PEG-based spacer. Its features enable robust conjugation under mild conditions while improving solubility and reducing steric constraints between binding elements. The subsequent points describe the structural attributes and practical reactivity considerations for constructing targeted protein degraders using this linker.
Structure: The linker contains a DBCO (dibenzocyclooctyne) moiety for strain-promoted cycloaddition, an amide linkage (NHCO) connecting to a polyethylene glycol spacer, and a biotin functional group. It features stable covalent bonds including amide and ether linkages, with PEG conferring hydrophilicity and flexible conformational behavior.
Reactivity: DBCO-based linkers react with azide-bearing partners via strain-promoted azide–alkyne cycloaddition, typically proceeding without added copper. Suitable conditions generally include aqueous or mixed aqueous/organic buffers at mild temperatures, with careful control of pH and ionic strength to preserve azide and biomolecule integrity. No special catalysts are required for the cycloaddition; purification is often performed by standard chromatographic or precipitation methods depending on the PROTAC format.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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