DBCO-NHCO-PEG6-Biotin
Cat No.: BP-502083
4.5 
DBCO-NHCO-PEG6-Biotin is a heterobifunctional PEG-based linker that combines a strained cyclooctyne (DBCO) for bioorthogonal azide–alkyne cycloaddition with an amide-linked biotin handle for high-affinity streptavidin capture. Structurally, it features a PEG6 spacer that provides aqueous solubility and rotational flexibility, while the NHCO group connects the DBCO functionality to the polymer chain without compromising the reactivity of the cyclooctyne. In PROTAC and targeted degradation workflows, such linkers are valuable for modular assembly and downstream conjugation: the DBCO moiety can be used to attach a PROTAC component (or a targeting/labeling partner) bearing an azide, enabling rapid, chemoselective coupling under mild conditions, and the biotin moiety allows affinity purification, immobilization, or detection of the resulting conjugate. This reagent supports efficient construct generation and analytical workflows, improving reproducibility in experiments that require controlled stoichiometry and robust purification of degradation-relevant molecules.
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* For research and manufacturing use only. Not for human or clinical use.
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This DBCO-NHCO-PEG6-Biotin linker is designed to support modular PROTAC construction by combining a strain-promoted azide–alkyne cycloaddition (SPAAC) handle with a biotin-functional tag. Its PEG-based spacer improves solubility and conformational flexibility, facilitating efficient conjugation and downstream target engagement workflows. The subsequent points describe its structural features and practical reactivity considerations for assembling PROTAC architectures using this linker.
Structure: The molecule contains a dibenzocyclooctyne (DBCO) cyclooctyne core for strain-promoted cycloaddition, an amide linkage (NHCO), and a poly(ethylene glycol) spacer that provides a flexible, hydrophilic chain. A biotin moiety is incorporated for affinity-based handling.
Reactivity: DBCO reagents react selectively with azides via SPAAC, typically proceeding under mild, catalyst-free conditions in aqueous or mixed aqueous solvents. For PROTAC assembly, this enables rapid ligation of azide-bearing warheads or linker fragments without copper, reducing potential metal-mediated side reactions. Reaction progress can be monitored by standard analytical methods, and purification is commonly performed using chromatographic or affinity-based approaches compatible with PEGylated conjugates.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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