DBCO-NHCO-PEG2-Biotin
Cat No.: BP-502106
4.5 
DBCО-NHCO-PEG2-Biotin is a bioconjugation-ready PROTAC linker featuring a DBCO (dibenzocyclooctyne) strained-alkyne handle connected through a short PEG spacer to a biotin moiety via a carbamate linkage. The PEG2 segment provides a flexible, hydrophilic spacer that helps reduce steric interference and improves accessibility of both the DBCO and biotin functionalities in aqueous conditions. In PROTAC or targeted degradation workflows, this linker is valuable for modular assembly and orthogonal conjugation: the DBCO group enables efficient strain-promoted azide–alkyne cycloaddition with azide-functionalized ligands or molecular handles, allowing researchers to “click” the linker into larger degradation constructs under mild conditions. The biotin end can further support affinity-based capture or localization using streptavidin-coated platforms, facilitating purification, immobilization, or analytical readouts. Overall, it supports streamlined synthesis and characterization of multi-component targeted protein degradation reagents where controlled conjugation and flexible spacing are critical.
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* For research and manufacturing use only. Not for human or clinical use.
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This DBCO-NHCO-PEG2-Biotin linker is designed for modular PROTAC assembly by combining a strain-promoted DBCO cyclooctyne handle with a biotin tag and a short PEG spacer. Its bifunctional architecture supports efficient conjugation under mild conditions, improving compatibility with sensitive targeting ligands and facilitating subsequent purification or detection workflows. The detailed Structure and Reactivity parameters are described below.
Structure: The linker contains a DBCO moiety for strain-promoted cycloaddition, an amide linkage (NHCO) connecting to a short ethylene glycol–based PEG segment, and a biotin-derived binding functionality. It features heteroatom-rich ether and amide groups that enhance solubility and provide conformational flexibility for linker presentation.
Reactivity: DBCO reacts rapidly with azide-functional partners via strain-promoted azide–alkyne cycloaddition, typically without added catalysts. Suitable conditions include aqueous or mixed aqueous/organic buffers at neutral to mildly basic pH, with careful control of solvent composition to maintain solubility. The mechanism proceeds through cycloaddition to form a stable triazole linkage, enabling straightforward conjugation of azide-bearing targeting ligands or PROTAC components.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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