Azido-C3-UV-biotin
Cat No.: BP-501797
4.5 
Azido-C3-UV-biotin (also known as UV Cleavable Biotin-PEG2-alkyne) is a biotin-containing cleavable linker bearing a terminal alkyne and a UV-cleavable moiety. The terminal alkyne enables chemoselective conjugation to azide-containing partners through copper-catalyzed azide–alkyne cycloaddition (CuAAC), allowing attachment to complementary PROTAC fragments or reporter groups. The UV-cleavable element enables photolytic release of the biotin-tagged conjugate under appropriate irradiation conditions, supporting controlled recovery after affinity enrichment. The biotin tag provides strong, high-specificity affinity enrichment for downstream pull-down assays, enabling sensitive detection and characterization of degraded targets or recruited proteins. This linker is valuable for mechanistic studies in targeted protein degradation, especially when researchers need UV-cleavable release together with affinity-based isolation of PROTAC-associated complexes.
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* For research and manufacturing use only. Not for human or clinical use.
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Azido-C3-UV-biotin is a versatile PROTAC linker building block designed to connect a photoactivatable UV handle with a biotin-based affinity element through a flexible C3 spacer and a terminal azide for modular conjugation. Its combination of orthogonal reactive functionalities enables efficient assembly of targeted protein degradation constructs and supports downstream validation workflows. The following sections describe its structural features and practical reactivity considerations for PROTAC synthesis in detail below.
Structure: The molecule contains an azide functional group suitable for bioorthogonal coupling, a flexible aliphatic C3 spacer that can modulate linker length and conformational freedom, and a UV-reactive moiety for photoinduced activation. A biotin-derived scaffold provides a strongly associating affinity handle, with heteroatom-rich functionality that supports solubility and conjugation.
Reactivity: The azide enables strain-promoted or copper-catalyzed azide–alkyne cycloaddition strategies, depending on the complementary partner used during PROTAC assembly. The UV-reactive component is typically engaged under controlled light exposure to drive photoactivation or crosslinking, following standard photochemical handling practices. Linker conjugations are commonly performed in inert or carefully degassed solvents compatible with both click chemistry and photoreactivity, using appropriate bases or catalysts only when required by the chosen coupling route.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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