Name: DBCO-PEG4-acid
Brand: BOC Sciences
Rating: 5 (1 reviews)

Size

Price

Stock

Quantity

$--

In stock

Purity

>95%

Solubility

In DMSO: 41.67 mg/mL (75.40 mM; Need ultrasonic)

Appearance

Transparent Liquid

Storage

Store at -20°C

Shipping

Room temperature in continental US; may vary elsewhere.

IUPACName

3-[2-[2-[2-[2-[[4-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-4-oxobutanoyl]amino]ethoxy]ethoxy]ethoxy]ethoxy]propanoic acid

Synonyms

DBCO-PEG4-C2-acid; DBCO-CONH-PEG4-acid

Boiling Point

839.7±65.0 °C at 760 mmHg

Density

1.27±0.1 g/cm3

InChI Key

LKSAMQQFMTVPKD-UHFFFAOYSA-N

InChI

InChI=1S/C30H36N2O8/c33-28(31-14-16-38-18-20-40-22-21-39-19-17-37-15-13-30(35)36)11-12-29(34)32-23-26-7-2-1-5-24(26)9-10-25-6-3-4-8-27(25)32/h1-8H,11-23H2,(H,31,33)(H,35,36)

SMILES

C1C2=CC=CC=C2C#CC3=CC=CC=C3N1C(=O)CCC(=O)NCCOCCOCCOCCOCCC(=O)O

ConcentrationVolumeMass	1 mg	5 mg	10 mg
1 mM	1.8096 mL	9.0478 mL	18.0956 mL
5 mM	0.3619 mL	1.8096 mL	3.6191 mL
10 mM	0.1810 mL	0.9048 mL	1.8096 mL

This DBCO-PEG4-acid linker is a bifunctional, click-reactive module designed for assembling PROTACs via strain-promoted azide–alkyne cycloaddition. Its PEG-based spacer supports productive conjugation while helping to reduce steric constraints between the targeting ligand and the E3-recruiting element. The terminal carboxylic acid enables reliable coupling to amines or other nucleophilic handles, facilitating modular PROTAC synthesis. Detailed structural and reactivity considerations are provided below.

Structure: DBCO-PEG4-acid comprises a cyclooctyne (DBCO) moiety connected through a polyethylene glycol spacer to a terminal carboxylic acid. It contains an alkyne within a strained ring system, ether linkages in the PEG chain, and a carboxylate functionality suitable for derivatization. Overall, it is amphiphilic and water-compatible due to the PEG segment.

Reactivity: The DBCO group undergoes strain-promoted azide–alkyne cycloaddition with azides under catalyst-free conditions, typically in aqueous or mixed aqueous buffers compatible with biomolecule conjugation. The carboxylic acid is suitable for amide-bond formation using standard coupling chemistries (for example, carbodiimide-based activation with an appropriate coupling additive) toward amines on ligands. Reaction design should consider pH and solubility to preserve linker integrity and conjugation efficiency.

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* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C₁V₁ = C₂V₂