Hydrazide-PEG4-Desthiobiotin
Cat No.: BP-501792
Purity: >95%
4.5 
Hydrazide-PEG4-Desthiobiotin is a heterobifunctional PEG-based linker designed for PROTAC and targeted degradation workflows that require stable conjugation to biotin-binding systems while retaining a reactive hydrazide handle for further coupling. Structurally, it comprises a short four-unit polyethylene glycol spacer that provides aqueous solubility and conformational flexibility, terminating in a desthiobiotin moiety for reversible capture by streptavidin or related biotin-binding proteins, and a hydrazide group that can form covalent bonds with compatible carbonyl-containing partners (e.g., aldehydes or activated carboxyl derivatives) under standard bioconjugation conditions. In PROTAC design, the PEG spacer helps decouple steric effects between the targeting module and the biotin-binding anchor, enabling efficient recruitment of the desired protein complex. Its value lies in facilitating modular assembly of degraders and related affinity reagents, supporting controlled localization, purification, and mechanistic studies of targeted protein degradation in biochemical and cellular experiments.
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* For research and manufacturing use only. Not for human or clinical use.
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Hydrazide-PEG4-Desthiobiotin is a functional linker incorporating a desthiobiotin affinity tag and a hydrazide handle for conjugation workflows; desthiobiotin is commonly used for avidin/streptavidin binding rather than as a general target- or E3-recruiting ligand. Its flexible ethylene glycol spacer improves conjugate solubility and conformational adaptability, while the hydrazide handle enables efficient formation of stable linkages to compatible warheads or targeting modules. The following sections describe its structure and practical reactivity considerations for PROTAC assembly in research settings.
Structure: The linker combines a hydrazide functional group with a short, flexible poly(ethylene glycol) spacer and a desthiobiotin motif. It contains amide and hydrazide-derived N–N and C–N linkages, along with ether linkages in the PEG segment, supporting aqueous compatibility and conformational flexibility.
Reactivity: The hydrazide group is suitable for coupling strategies that form stable amide or hydrazone-type intermediates with appropriately activated carbonyl partners, typically under mild, aqueous-compatible conditions. Common approaches include condensation with aldehydes or use of activated carboxylic acid derivatives to generate amide bonds. Solvents such as buffered aqueous mixtures and polar aprotic media are often used, with pH control to balance coupling efficiency and minimize side reactions.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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