Pyrene-PEG5-biotin
Cat No.: BP-501790
Purity: 97%
4.5 
Pyrene-PEG5-biotin is a heterobifunctional linker featuring a pyrene fluorophore conjugated to a short polyethylene glycol chain terminated with biotin. Structurally, the PEG spacer provides water solubility and conformational flexibility, while the pyrene moiety enables sensitive hydrophobic π–π interactions and fluorescence-based tracking of the linker’s location in membranes or hydrophobic microenvironments. The biotin end serves as a high-affinity handle for streptavidin or avidin binding, allowing robust, modular attachment of the linker to biotinylated ligands or protein scaffolds. In PROTAC and targeted degradation workflows, this type of linker is valuable for constructing experimentally tractable conjugates where one component must be positioned or monitored while the other is presented to an affinity capture system. Its combination of fluorescence readout and strong biotin–avidin coupling supports mechanistic studies of conjugate assembly, cellular uptake, and ternary-complex formation.
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* For research and manufacturing use only. Not for human or clinical use.
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Pyrene-PEG5-biotin is a multifunctional PROTAC linker component designed to combine a hydrophobic pyrene tag with a flexible polyethylene glycol spacer and a biotin handle for affinity-based workflows. Its modular architecture supports conjugation strategies that enable controlled assembly of targeted protein degraders and facilitates downstream characterization or capture. The points below describe the structural features and practical reactivity considerations for constructing PROTACs using this linker.
Structure: The linker contains a pyrene aromatic moiety connected to a PEG chain and a biotin-derived bioconjugation site. It features ether linkages within the PEG segment, an aromatic system for π-interactions, and amide/urea-type functionalities typical of biotin derivatives. Overall, it is an amphiphilic, water-compatible conjugation scaffold.
Reactivity: Suitable PROTAC construction typically relies on orthogonal bioconjugation chemistries that couple the biotin end to biotin-recognizing capture systems or to biotin-functionalized building blocks, while the pyrene/PEG region remains available for noncovalent or tagging applications. Use reaction conditions compatible with PEG stability and biotin integrity, commonly employing mild aqueous buffers or mixed solvents, with coupling reagents selected to preserve amide/ether bonds and avoid harsh hydrolysis.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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