Biotin-PEG3-Mal
CAS No.: 1431618-70-0
Cat No.: BP-501393
Purity: >97%
4.5 
Biotin-PEG3-Mal is a heterobifunctional PROTAC linker consisting of a biotin moiety connected through a short, flexible polyethylene glycol spacer to a maleimide functional group. The PEG3 segment provides aqueous solubility and conformational flexibility, while the maleimide enables chemoselective conjugation to thiol-containing partners (for example, cysteine residues or thiol-terminated ligands) via a stable thioether linkage. In targeted protein degradation workflows, this linker can be used to attach a degraders’ targeting or E3-recruiting component to thiol-functionalized molecules, or to incorporate biotin handles for affinity capture and downstream analytical enrichment. Its short PEG chain helps maintain effective proximity between conjugated modules, supporting efficient formation of the final construct and facilitating purification, pull-down assays, and mechanistic studies in PROTAC and related targeted degradation research.
Structure of 1431618-70-0
Assurance
Delivery
Support
* For research and manufacturing use only. Not for human or clinical use.
| Size | Price | Stock | Quantity |
|---|---|---|---|
| -- | $-- | In stock |
Looking for different specifications? Click to request a custom quote!
Capabilities & Facilities
- Comprehensive PROTAC Platform
- Scientific Expertise & Technical Support
- Custom Synthesis & Design Service
- Extensive Product Coverage
- Cutting-Edge Innovation
- Fast Delivery & Global Support
- 24/7 customer service
- 100% quality assurance
Popular Publications Citing BOC Sciences Products
This biotin-PEG3-maleimide linker is designed for modular PROTAC construction and conjugation workflows that benefit from strong biotin-mediated handling and a flexible polyethylene glycol spacer. The maleimide functionality enables efficient coupling to thiol-bearing ligands, while the PEG segment supports solubility and reduces steric constraints during ternary complex formation. Detailed structural and reactivity considerations for experimental assembly are provided below.
Structure: The linker combines a biotin headgroup with a short polyethylene glycol chain and a terminal maleimide electrophile. It contains heteroatom-rich ether linkages, an imide ring, and a thio-Michael acceptor double bond. The PEG segment imparts hydrophilicity and conformational flexibility, improving aqueous compatibility.
Reactivity: The maleimide group reacts with sulfhydryl-containing partners via a thiol–maleimide (thio-Michael) addition, typically under mildly basic to neutral aqueous conditions. Use fresh thiols or thiol-containing biomolecules, and avoid strong nucleophiles and reducing agents that can compete with maleimide. The reaction is generally performed in buffered aqueous solvent, where pH and thiol availability govern coupling efficiency and selectivity.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
Related Product Recommendations
Please contact us with any specific requirements and we will get back to you as soon as possible.
