Biotin-PEG9-NHS Ester
Cat No.: BP-502067
4.5 
Biotin-PEG9-NHS Ester is a heterobifunctional PEG-based linker that combines a biotin affinity handle with an N-hydroxysuccinimide (NHS) ester for covalent coupling to primary amines. Structurally, it contains a biotin moiety connected through a polyethylene glycol chain of approximately nine ethylene glycol units, providing water solubility and spatial flexibility, while the NHS ester enables rapid formation of stable amide bonds under standard amine-reactive labeling conditions. In PROTAC and targeted degradation workflows, this reagent is valuable for attaching biotin-tagged probes or affinity handles to lysine-containing ligands, E3 ligase binders, or other targeting components, thereby facilitating pull-down, detection, and stoichiometry studies without requiring modification of the core binding pharmacophore. Its PEG spacer helps preserve binding interactions by reducing steric interference, making it a practical tool for experimental optimization, characterization, and mechanistic assays in targeted protein degradation research.
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* For research and manufacturing use only. Not for human or clinical use.
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Biotin-PEG9-NHS Ester is a PEG-based biotinylation linker designed for efficient conjugation of amine-containing ligands to NHS-activated surfaces or intermediates used in PROTAC workflows. Its flexible polyethylene glycol spacer supports favorable linker solubility and reduces steric interference between the targeting ligand and the recruitment module. The biotin handle enables affinity-based detection or purification, while the NHS ester provides a widely used, reliable route to stable amide bond formation. Detailed structural and reactivity considerations are provided below.
Structure: The molecule comprises a biotin moiety connected through a flexible polyethylene glycol chain to an N-hydroxysuccinimide ester. It contains an activated carboxylate (NHS ester) suitable for nucleophilic acyl substitution, along with ether linkages typical of PEG. Overall, it is designed for aqueous compatibility and controlled hydrophilicity.
Reactivity: The NHS ester reacts with primary amines to form stable amide bonds via nucleophilic acyl substitution, typically under mildly basic conditions to promote amine deprotonation while preserving NHS reactivity. Common approaches use aqueous buffers or mixed organic/aqueous solvents, with careful control of pH and reaction time to minimize hydrolysis. No special catalysts are generally required; the reaction proceeds through the activated ester intermediate.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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