Biotin-C10-NHS Ester
CAS No.: 887628-40-2
Cat No.: BP-501688
4.5 
Biotin-C10-NHS Ester is a biotinylated, ten-carbon (C10) linker bearing an N-hydroxysuccinimide (NHS) ester, providing a reactive acylating group for stable amide bond formation with primary amines on proteins, peptides, or amine-functionalized PROTAC components. Structurally, the long aliphatic spacer separates the biotin moiety from the conjugation site, improving accessibility for streptavidin/avidin binding and thereby enabling efficient affinity capture and detection. In PROTAC design and targeted degradation workflows, this linker is useful as a modular “handle” to attach biotin to one partner of a heterobifunctional construct, facilitating pull-down assays, immobilization on streptavidin-coated surfaces, and quantitative analysis of binding or ternary complex formation. Its value lies in supporting rigorous biochemical characterization and purification steps, helping researchers validate conjugation efficiency and monitor component engagement during PROTAC optimization.
Structure of 887628-40-2
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Biotin-C10-NHS Ester is a biotinylated, NHS-activated linker designed for efficient coupling in PROTAC workflows where a stable handle for affinity-based detection, enrichment, or modular conjugation is advantageous. Its activated ester enables straightforward formation of amide bonds with primary amines under mild conditions, supporting reliable linker installation and subsequent PROTAC assembly. The sections below describe the linker’s structure and practical reactivity considerations in detail.
Structure: The linker contains a biotin moiety connected through a C10 alkyl spacer to an N-hydroxysuccinimide (NHS) ester. It features an amide-forming activated carboxylate, stable aliphatic C–C connectivity, and ester functionality that is reactive toward nucleophiles. Overall, it is a hydrophobic, spacer-rich conjugation reagent.
Reactivity: NHS ester chemistry is typically used to couple to primary amines via nucleophilic acyl substitution, forming a stable amide linkage. Suitable conditions involve mildly basic aqueous or mixed aqueous-organic buffers to maintain amine nucleophilicity while limiting hydrolysis. Reactions are commonly performed without strong nucleophiles; catalysts are generally unnecessary. After coupling, unreacted ester is removed by standard purification, and the resulting amide-conjugate can be carried forward into PROTAC construct preparation.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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