Biotin-PEG2-C4-alkyne
CAS No.: 1011268-28-2
Cat No.: BP-501047
4.5 
Biotin-PEG2-C4-alkyne is a bifunctional PROTAC linker building block combining a biotin affinity handle with a short polyethylene glycol (PEG) spacer and a terminal alkyne for orthogonal conjugation chemistry. The PEG2 segment provides aqueous solubility and conformational flexibility, while the C4 alkyl spacer positions the reactive alkyne away from the biotin moiety to reduce steric interference during coupling. In targeted protein degradation workflows, this linker can be used to install biotin tags on degraders or related intermediates, enabling affinity enrichment, pull-down validation, and mechanistic studies of ternary-complex formation when paired with biotin-binding reagents. The terminal alkyne supports widely used click-type or alkyne-reactive conjugation strategies to connect the linker to complementary azide- or alkyne-bearing partners, facilitating modular PROTAC assembly. Overall, it is valuable for researchers seeking robust, experimentally tractable biotinylated constructs for characterization and optimization of targeted degradation systems.
Structure of 1011268-28-2
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Biotin-PEG2-C4-alkyne is a bifunctional PROTAC linker designed to connect a biotin-based recognition handle to an alkyne-bearing coupling site, enabling modular assembly of targeted protein degraders. Its polyethylene glycol segment supports aqueous compatibility and flexible spacing, while the terminal alkyne provides a versatile chemical “handle” for conjugation. The linker is commonly applied in PROTAC workflows where researchers require reliable, orthogonal attachment strategies; the following sections describe its structure and practical reactivity considerations in detail below.
Structure: The linker contains a biotin-derived motif linked through a short polyethylene glycol spacer to a C4 tether terminating in a terminal alkyne. It features ether linkages from the PEG segment, amide/heteroatom-rich connectivity typical of biotin derivatives, and a carbon–carbon triple bond suitable for click-type coupling.
Reactivity: The terminal alkyne enables propargyl-compatible conjugation, most notably copper-catalyzed azide–alkyne cycloaddition for assembling PROTAC constructs with azide-functional partners. Typical conditions use a copper(I) source, an appropriate ligand system, and polar solvents compatible with PEG-containing linkers; mild temperatures and inert atmosphere are often employed to minimize side reactions. Alternative alkyne-reactive chemistries can be used when azide–alkyne coupling is not desired, following established coupling principles for alkynes.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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