Biotin-PEG3-SH
CAS No.: 1244028-52-1
Cat No.: BP-501235
4.5 
Biotin-PEG3-SH is a biotin-functionalized polyethylene glycol linker bearing a terminal thiol, providing a short, flexible PEG3 spacer between the biotin moiety and a reactive sulfhydryl group. Structurally, it combines the high-affinity biotin–streptavidin interaction with a hydrophilic PEG segment that helps reduce nonspecific adsorption and improves solubility in aqueous PROTAC-related workflows. In targeted protein degradation design, this linker is useful as a conjugation handle for attaching biotinylated probes, affinity capture tags, or immobilization-compatible PROTAC components to streptavidin-coated surfaces or for assembling multi-component degradation systems. The terminal thiol enables chemoselective coupling to maleimide-activated partners or other thiol-reactive chemistries, facilitating modular construction and purification. Overall, it supports rigorous experimental workflows by enabling controlled conjugation and convenient affinity-based enrichment of biotinylated constructs used to study ternary complex formation, cellular uptake, and degradation kinetics.
Structure of 1244028-52-1
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Biotin-PEG3-SH is a functionalized polyethylene glycol-based linker designed to support PROTAC assembly by providing a biotin handle for affinity-guided workflows and a terminal thiol for chemoselective conjugation. Its PEG-rich segment promotes aqueous solubility and flexible spacing between binding elements, which can help preserve productive ternary complex formation. The linker’s structure and reactivity are described in detail below to support researchers in designing and optimizing targeted protein degradation constructs.
Structure: Biotin-PEG3-SH contains a biotin moiety connected through a polyethylene glycol chain terminating in a free thiol. The molecule features ether linkages within the PEG segment and a thiol functional group capable of forming thioether or disulfide linkages. Overall, it is typically water-compatible and conformationally flexible.
Reactivity: The terminal thiol enables conjugation via thiol-selective chemistries commonly used in PROTAC linker construction, including maleimide-thiol addition, haloacetamide alkylation to form thioethers, and controlled disulfide exchange for reversible designs. Reactions are generally conducted under mild, thiol-preserving conditions in suitable aqueous or mixed solvents, often with pH control and exclusion of strong oxidants; catalysts are not typically required for standard thiol–electrophile coupling.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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