Biotin-PEG12-TFP ester
Cat No.: BP-501805
4.5 
Biotin-PEG12-TFP ester is a heterobifunctional PROTAC linker built on a polyethylene glycol chain of moderate length, terminated with a biotin handle and a reactive tetrafluorophenyl (TFP) ester. Structurally, the PEG segment provides aqueous solubility and conformational flexibility, while the biotin moiety enables strong, high-affinity capture or pre-localization on streptavidin/avidin-functionalized platforms. The TFP ester acts as an activated acylating group that readily reacts with primary amines on proteins, peptides, or amine-bearing small molecules to form stable amide linkages. In targeted protein degradation workflows, this linker is useful for modular assembly of conjugates, including attachment of targeting ligands or E3 ligase binders to biomolecular carriers, and for purification or immobilization steps that improve experimental throughput and reproducibility. Its flexible PEG scaffold and robust biotin–avidin chemistry make it a practical tool for designing and testing degradation constructs.
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* For research and manufacturing use only. Not for human or clinical use.
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Biotin-PEG12-TFP ester is a heterobifunctional PROTAC linker designed to connect a biotin-bearing handle with a highly reactive TFP (tetrafluorophenyl) ester for efficient conjugation to primary amines on ligands. Its PEG spacer provides water compatibility and conformational flexibility, supporting productive ternary complex formation. The TFP ester chemistry enables reliable coupling under standard bioconjugation workflows; detailed structural and reactivity considerations are provided below.
Structure: The molecule combines a biotin moiety with a polyethylene glycol spacer and a terminal TFP ester. It contains ester and ether linkages, aromatic tetrafluorophenyl functionality, and multiple heteroatoms that enhance polarity. The PEG segment confers hydrophilicity and conformational flexibility relevant to linker behavior in aqueous media.
Reactivity: The TFP ester is suited for acylation of primary amines to form stable amide bonds, a common strategy in PROTAC assembly. Conjugations are typically performed in mildly basic aqueous or mixed solvent conditions to promote nucleophilic attack by amine-containing ligands, with the TFP leaving group facilitating efficient coupling. Solvents such as aqueous buffers with compatible cosolvents are commonly used; purification by standard chromatographic or dialysis methods follows to remove hydrolyzed ester byproducts.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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