Biotin-PEG24-NHS ester
Cat No.: BP-502074
4.5 
Biotin-PEG24-NHS ester is a heterobifunctional PEG-based linker combining a biotin affinity handle with an N-hydroxysuccinimide (NHS) ester for covalent coupling to primary amines. Structurally, it consists of a long, flexible polyethylene glycol chain that spaces the reactive NHS group from the biotin moiety, enabling efficient conjugation while minimizing steric interference during downstream assays. In PROTAC and targeted protein degradation workflows, this linker is useful for attaching biotin-tagged probes or affinity reagents to amine-containing components, such as lysine-bearing ligands, E3 ligase recruiters, or linker intermediates, facilitating purification, immobilization, and pull-down experiments that verify ternary complex formation or degradation engagement. Its PEG architecture improves aqueous solubility and reduces nonspecific interactions, supporting robust experimental handling and reproducible characterization of conjugates. Overall, it provides a practical biotin-enabled tool for mechanistic studies and workflow optimization in targeted degradation research.
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* For research and manufacturing use only. Not for human or clinical use.
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Biotin-PEG24-NHS ester is a heterobifunctional PEG-based linker designed to connect biotin-tagging handles with amine-reactive NHS chemistry, enabling modular PROTAC and related targeted degradation workflows. Its flexible poly(ethylene glycol) spacer supports productive spatial presentation between binding and recruiting elements, while the NHS ester provides a robust route for forming stable amide bonds under mild conditions. The detailed Structure and Reactivity parameters are provided below.
Structure: The linker comprises a biotin moiety tethered to a long, flexible PEG chain terminated by an N-hydroxysuccinimide ester. It contains ester and amide-forming functional groups, with ether linkages within the PEG segment. The overall scaffold is polar, water-compatible, and conformationally flexible, supporting linker-mediated distance control.
Reactivity: The NHS ester reacts with primary amines to form amide bonds via nucleophilic acyl substitution. Suitable conditions typically use mildly basic aqueous buffers or organic/aqueous mixtures to maintain amine nucleophilicity while minimizing NHS hydrolysis. No special catalysts are generally required; careful pH control and fresh preparation improve coupling efficiency. Common solvents include water and alcohol-containing buffers, and purification is often performed after conjugation to remove hydrolyzed NHS byproducts.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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