Biotin-PEG12-hydrazide
Cat No.: BP-501825
4.5 
Biotin-PEG12-hydrazide is a PEG-based heterobifunctional linker that combines a terminal biotin handle with a hydrazide reactive group for covalent conjugation to carbonyl-containing partners. Structurally, it features a biotin moiety connected through a long, flexible polyethylene glycol spacer, followed by a hydrazide at the opposite end, enabling both affinity-based capture (via biotin–streptavidin or biotin-binding proteins) and chemical coupling through hydrazone formation with aldehydes or ketones. In PROTAC and targeted protein degradation workflows, such linkers are valuable for attaching or positioning recognition and payload elements while maintaining solubility and reducing steric interference through the PEG chain. The hydrazide functionality supports controlled attachment of degraders, probes, or linker intermediates to carbonyl-bearing components, and the biotin tag facilitates analytical readouts, pull-down experiments, and surface immobilization during optimization. Overall, it provides a practical modular tool for constructing and characterizing targeted degradation conjugates with improved handling and experimental flexibility.
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* For research and manufacturing use only. Not for human or clinical use.
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Biotin-PEG12-hydrazide is a polyethylene glycol-based linker that combines a biotin affinity motif with a nucleophilic hydrazide handle for conjugation to aldehyde/ketone partners or activated acyl electrophiles. Its flexible PEG segment improves solubility and provides spacing, while the hydrazide functionality enables hydrazone formation or reaction with complementary activated acyl electrophiles. The following points describe its structure and practical reactivity considerations for PROTAC assembly in detail below.
Structure: The linker contains a PEG chain providing a flexible, hydrophilic scaffold, terminating in a hydrazide group for nucleophilic acyl substitution chemistry. A biotin-derived moiety supports strong affinity interactions, while the carbonyl and N–N bonds provide defined hydrogen-bonding and reactivity sites.
Reactivity: The hydrazide can participate in acyl-transfer and hydrazone-forming conjugation workflows, making it suitable for constructing PROTACs via formation of hydrazone linkages under mildly acidic conditions or via coupling to complementary electrophiles bearing activated carbonyls. Typical approaches use polar organic solvents or aqueous/organic mixtures, with acid catalysis for hydrazone formation and standard peptide/amide coupling reagents when converting the hydrazide to targeted intermediates.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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