Biotin-PEG5-amine
CAS No.: 113072-75-6
Cat No.: BP-501417
Purity: >95%
4.5 
Biotin-PEG5-amine is a heterobifunctional PEG-based linker that combines a biotin affinity handle with a terminal primary amine for covalent coupling to PROTAC-relevant ligands or to activated groups on other components. The structure features a biotin moiety connected through a short, flexible polyethylene glycol chain (five ethylene glycol units), providing water solubility and conformational spacing that can reduce steric interference during ternary-complex formation. In targeted protein degradation workflows, this linker enables researchers to attach biotin-tagged probes or to incorporate biotin-mediated capture steps for monitoring synthesis, purification, or cellular engagement of PROTAC assemblies. The terminal amine supports standard amide or urea coupling chemistries with carboxyl- or isocyanate-activated partners, facilitating modular PROTAC design. Overall, it is valuable for constructing affinity-enabled degradation reagents and for enabling reproducible biochemical assays that track conjugate integrity and binding behavior.
Structure of 113072-75-6
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Biotin-PEG5-amine is a polyethylene glycol (PEG)-based linker designed to connect biotin-containing recognition elements to amine-reactive coupling partners in targeted protein degradation workflows. Its hydrophilic PEG spacer supports solubility and can help tune the spatial presentation of ligands for efficient ternary complex formation. The amine functionality enables straightforward conjugation chemistry, making it a practical building block for constructing PROTACs and related targeted degradation constructs. Detailed structural and reactivity considerations are provided below.
Structure: The linker contains a PEG chain terminating in a primary amine and a biotin-derived recognition handle. It features ether linkages within the PEG segment, providing conformational flexibility and hydrophilicity, while the terminal amine offers a reactive nucleophile for bond formation with activated electrophiles.
Reactivity: The terminal primary amine can be used in amide bond formation with activated carboxylic acids (for example, NHS esters or activated acid derivatives) under standard coupling conditions. Alternatively, it can participate in reductive amination with aldehyde-bearing partners in the presence of suitable reducing agents. Mild bases and polar aprotic solvents are commonly employed to maintain linker integrity and promote efficient conjugation.
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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